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来自热液喷口管虫厚壳贻贝细菌共生体的II型核酮糖-1,5-二磷酸羧化酶/加氧酶的克隆与测序

Cloning and sequencing of a form II ribulose-1,5-biphosphate carboxylase/oxygenase from the bacterial symbiont of the hydrothermal vent tubeworm Riftia pachyptila.

作者信息

Robinson J J, Stein J L, Cavanaugh C M

机构信息

Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138, USA.

出版信息

J Bacteriol. 1998 Mar;180(6):1596-9. doi: 10.1128/JB.180.6.1596-1599.1998.

Abstract

The bacterial symbiont of the hydrothermal vent tubeworm fixes carbon via the Calvin-Benson cycle and has been shown previously to express a form II ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO). The gene cbbM, which encodes this enzyme, has been cloned and sequenced. The gene has the highest identity with the cbbM gene from Rhodospirillum rubrum, and analysis of the inferred amino acid sequence reveals that all active-site residues are conserved. This is the first form II RubisCO cloned and sequenced from a chemoautotrophic symbiont and from a deep-sea organism.

摘要

热液喷口管蠕虫的细菌共生体通过卡尔文-本森循环固定碳,并且先前已证明其表达II型核酮糖-1,5-二磷酸羧化酶/加氧酶(RubisCO)。编码该酶的cbbM基因已被克隆和测序。该基因与来自深红红螺菌的cbbM基因具有最高的同源性,对推导的氨基酸序列分析表明所有活性位点残基都是保守的。这是首次从化学自养共生体和深海生物中克隆和测序的II型RubisCO。

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