Rady P L, Hodak E, Yen A, Memar O, Trattner A, Feinmesser M, David M, Hudnall S D, Tyring S K
Department of Pediatrics, The University of Texas Medical Branch, Galveston 77555-1019, USA.
J Am Acad Dermatol. 1998 Mar;38(3):429-37. doi: 10.1016/s0190-9622(98)70501-8.
Kaposi's sarcoma (KS) accounts for more than 5% of malignancies in immunosuppressed organ transplant patients (OKS). A new herpesvirus (HHV-8) was identified with high prevalence in biopsy specimens of AIDS-KS, endemic KS, and classic KS and in OKS. KS has also been associated with other underlying diseases in patients treated with corticosteroids, but this subset of KS has been reported to contain HHV-8 in only a few case reports.
In this larger study, we determined the prevalence of HHV-8 in seven patients of Jewish origin in whom KS developed during immunosuppressive therapy for different primary diseases (ISKS).
The study included HHV-8 DNA detection by polymerase chain reaction (PCR) coupled with Southern blot and sequence analysis as well as by in situ hybridization.
HHV-8 sequences were detected by PCR with confirmation by Southern blot and sequence analysis in 100% of the ISKS samples. Direct sequencing revealed several previously unknown base changes within the 208 bp region from open reading frame 26 (ORF26[208]) of HHV-8 in ISKS.
Ours is the largest known study describing the presence of HHV-8 in iatrogenic KS from immunosuppressed nontransplant patients and provides data of previously unknown sequence variations within the ORF26 of HHV-8 DNA.
卡波西肉瘤（KS）在免疫抑制的器官移植患者（OKS）的恶性肿瘤中占比超过5%。一种新的疱疹病毒（HHV-8）在艾滋病相关卡波西肉瘤、地方性卡波西肉瘤和经典型卡波西肉瘤的活检标本以及OKS中被发现具有高流行率。KS也与接受皮质类固醇治疗的患者的其他基础疾病有关，但仅在少数病例报告中报道过这一亚型的KS含有HHV-8。
在这项规模更大的研究中，我们确定了7名犹太裔患者中HHV-8的流行率，这些患者在针对不同原发性疾病的免疫抑制治疗期间发生了KS（ISKS）。
该研究包括通过聚合酶链反应（PCR）结合Southern印迹和序列分析以及原位杂交来检测HHV-8 DNA。
通过PCR检测到HHV-8序列，并经Southern印迹和序列分析在100%的ISKS样本中得到证实。直接测序揭示了ISKS中HHV-8开放阅读框26（ORF26[208]）的208 bp区域内几个先前未知的碱基变化。
我们的研究是已知最大规模的描述免疫抑制非移植患者医源性KS中HHV-8存在情况的研究，并提供了HHV-8 DNA的ORF26内先前未知的序列变异数据。
