Jaworski M D, Hunter D L, Ward A C
University of Idaho, Caine Veterinary Teaching and Research Center, Caldwell 83605, USA.
J Vet Diagn Invest. 1998 Jan;10(1):49-55. doi: 10.1177/104063879801000109.
A total of 608 bacterial isolates previously identified as Pasteurella haemolytica biotypes A and 3, P. trehalosi, and P. multocida, were separated into 73 distinct biovariants using 21 phenotypic characteristics. The largest group (54%) of wildlife isolates was identified as biogroup 2 and biogroup 2 variants. Biogroup 2 and biogroup 2 variants accounted for only 17% of isolates from domestic ruminants and were all from sheep. In contrast, 43% of isolates from domestic ruminants were identified as biogroup 1 and biogroup 1 variants, whereas only 6% of isolates from wildlife were identified in these groups. The majority of biogroup 1 isolates from wild ruminants were from 1 group of bighorn sheep in Arizona that were geographically separated from other wildlife sampled. Similarly, 1 biogroup 2 variant, 2E, was cultured only from free-ranging Dall sheep in Alaska. Twelve percent of domestic isolates and 6% of wildlife isolates were indole positive. The remaining isolates from wildlife (33%) and domestic animals (30%) were distributed among 53 distinct biovariants. None of these individual biovariants represented >4% of the total isolates. Phenotypic characterization was valuable for distinguishing between isolates from different hosts and from different geographic areas and can be used to assist in epidemiologic studies.
总共608株先前鉴定为溶血巴斯德氏菌生物型A和3、海藻糖巴斯德氏菌及多杀巴斯德氏菌的细菌分离株，利用21种表型特征被分为73个不同的生物变种。野生动物分离株中最大的组（54%）被鉴定为生物群2及生物群2变种。生物群2及生物群2变种仅占来自家养反刍动物分离株的17%，且均来自绵羊。相比之下，来自家养反刍动物的分离株中有43%被鉴定为生物群1及生物群1变种，而来自野生动物的分离株中只有6%属于这些组。来自野生反刍动物的生物群1分离株大多来自亚利桑那州的一群大角羊，这群羊在地理上与其他采样的野生动物分开。同样，1个生物群2变种，即2E，仅从阿拉斯加的野生多尔绵羊中培养得到。12%的家养分离株和6%的野生动物分离株吲哚呈阳性。其余来自野生动物的分离株（33%）和家畜的分离株（30%）分布在53个不同的生物变种中。这些单个生物变种中没有一个占总分离株的比例超过4%。表型特征对于区分来自不同宿主和不同地理区域的分离株很有价值，可用于辅助流行病学研究。
