Beck C W, Slack J M
Developmental Biology Programme, School of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK.
Mech Dev. 1998 Mar;72(1-2):41-52. doi: 10.1016/s0925-4773(98)00015-x.
We have studied Xenopus tail development from the end of gastrulation to the commencement of outgrowth at the tail bud stage. We show that an early group of genes are expressed at the stage of tail bud determination, at the end of gastrulation, and a late group are expressed at around stage 27 just before tail bud outgrowth. Together, these genes define seven distinct regions of the tail bud as outgrowth commences. We have previously shown that formation of a tail bud depends on the interaction of three tissue regions, called N, M and C, at stage 13. Here we show that expression of the late group of genes is dependent on this NMC interaction. We describe molecular correlates of two of these regions, M and C, which were formerly unobservable and whose existence was inferred from embryological experiments.
我们研究了非洲爪蟾从原肠胚形成末期到尾芽期开始长出尾巴这一阶段的尾巴发育过程。我们发现，一组早期基因在原肠胚形成末期的尾芽确定阶段表达，而一组晚期基因在尾芽长出前的大约第27阶段表达。随着尾巴开始长出，这些基因共同界定了尾芽的七个不同区域。我们之前已经表明，尾芽的形成依赖于第13阶段三个组织区域（称为N、M和C）的相互作用。在此我们表明，晚期基因的表达依赖于这种NMC相互作用。我们描述了其中两个区域M和C的分子关联，这两个区域以前无法观察到，其存在是从胚胎学实验中推断出来的。
