Prakash P, Leykin L, Chen Z, Toth T, Sayegh R, Schiff I, Isaacson K
Vincent Andrology and In Vitro Fertilization Laboratories, Massachusetts General Hospital, Harvard Medical School, Boston, USA.
Fertil Steril. 1998 Apr;69(4):722-6. doi: 10.1016/s0015-0282(98)00002-8.
To evaluate two commonly used methods of sperm preparation with respect to their effects on sperm morphology (strict criteria).
Auto-controlled, split sample study performed on the semen of 74 male partners of couples enrolled for IVF.
In vitro fertilization and andrology laboratories at a tertiary care, major teaching hospital.
PATIENT(S): Seventy-four male partners of couples who were scheduled to undergo IVF.
INTERVENTION(S): Equal halves of the same semen sample were evaluated for strict criteria sperm morphology before and after preparation by differential gradient centrifugation using Percoll (Pacific Andrology, Montrose, CA) and by the standard swim-up method.
MAIN OUTCOME MEASURE(S): The percentage of morphologically normal sperm was assessed using strict criteria before and after the two methods of sperm preparation. Specific parameters studied were individual abnormalities of the head, midpiece, and tail.
RESULT(S): Sperm preparation using differential gradient centrifugation with Percoll produced a significantly greater number of specimens with normal sperm morphology and also showed higher absolute quantitative improvement over the swim-up method. The two methods were comparable in regard to their effects on specific sperm abnormalities (i.e., head, midpiece, and tail defects).
CONCLUSION(S): The differential gradient sperm separation method using Percoll is superior to the swim-up method for selecting sperm with normal morphology as assessed by strict criteria. Because sperm morphology as assessed by strict criteria is a good predictor of oocyte fertilization, this method can be recommended as the method of choice for assisted reproductive technology laboratories. Use of this method may help improve outcome by increasing fertilization rates.
评估两种常用的精子制备方法对精子形态（严格标准）的影响。
对74对接受体外受精（IVF）夫妇的男性伴侣的精液进行自身对照、样本分割研究。
一家三级医疗、大型教学医院的体外受精实验室和男科学实验室。
74对计划接受IVF的夫妇的男性伴侣。
同一精液样本的等分样本在使用Percoll（太平洋男科学公司，加利福尼亚州蒙特罗斯）通过密度梯度离心法和标准上游法制备前后，根据严格标准评估精子形态。
在两种精子制备方法前后，使用严格标准评估形态正常精子的百分比。研究的具体参数是头部、中段和尾部的个体异常情况。
使用Percoll密度梯度离心法制备精子产生形态正常精子的标本数量显著更多，并且与上游法相比在绝对定量改善方面也更高。两种方法在对特定精子异常（即头部、中段和尾部缺陷）的影响方面具有可比性。
使用Percoll的密度梯度精子分离法在通过严格标准评估选择形态正常精子方面优于上游法。由于通过严格标准评估的精子形态是卵母细胞受精的良好预测指标，该方法可推荐为辅助生殖技术实验室的首选方法。使用该方法可能通过提高受精率来帮助改善结局。
