Hattel A L, Castro M D, Gummo J D, Weinstock D, Reed J A, Dubey J P
Animal Diagnostic Laboratory, Pennsylvania State University, University Park 16802, USA.
Vet Parasitol. 1998 Jan 31;74(2-4):307-13. doi: 10.1016/s0304-4017(97)00158-1.
Neospora caninum was found in fetal tissues of 34 of 688 cases of bovine abortion submitted to the Pennsylvania Animal Diagnostic Laboratory System during the period from May 1994 to November 1996. The aborted fetuses ranged in gestational age from 3 to 8 months. Microscopic lesions consisted primarily of encephalitis and myocarditis. A labeled (strept) avidin-biotin staining procedure using anti-N. caninum polyclonal rabbit serum revealed N. caninum organisms within the fetal brain (27 of 27), heart (10 of 13), placenta (5 of 6), kidney (2 of 2), liver (1 of 4) and skeletal muscle (1 of 1).
1994年5月至1996年11月期间，提交给宾夕法尼亚动物诊断实验室系统的688例牛流产病例中，有34例的胎儿组织中发现了犬新孢子虫。流产胎儿的胎龄为3至8个月。显微镜下病变主要为脑炎和心肌炎。使用抗犬新孢子虫多克隆兔血清的标记（链）抗生物素蛋白-生物素染色程序显示，在胎儿脑（27例中的27例）、心脏（13例中的10例）、胎盘（6例中的5例）、肾脏（2例中的2例）、肝脏（4例中的1例）和骨骼肌（1例中的1例）中存在犬新孢子虫。
