Walton L J, Macey M G, Thornhill M H, Farthing P M
Department of Oral Pathology, St Bartholomew's and the Royal London School of Medicine and Dentistry, England.
J Oral Pathol Med. 1998 Mar;27(3):116-23. doi: 10.1111/j.1600-0714.1998.tb01926.x.
This study has addressed the question of whether there is selective recruitment and distribution of intra-epithelial leucocytes in lesions of oral lichen planus (OLP). T-lymphocyte subsets were examined in the epithelium and peripheral blood of patients and controls using flow cytometry and double immunofluorescence, and the relationship between keratinocyte intercellular adhesion molecule-1 (ICAM-1) expression with T-lymphocyte and Langerhans cell (LC) distribution was examined. The circulating 'memory' subset (CD45RO+) of T-helper cells (CD4+) was increased from 49.1% in controls to 65.7% in patients (P=0.005), while the 'naive' subset (CD45RA+), which was absent from control epithelium, comprised 24% of helper cells in OLP (P=0.016). Fewer LC expressed CD45RO in OLP than in controls (P=0.037) and all T-cell and LC counts were significantly raised in ICAM-1-expressing areas of epithelium. These data demonstrate changes in intra-epithelial T-lymphocyte and LC populations compared with normal oral mucosa and suggest there is selective recruitment in OLP. In addition, keratinocyte ICAM-1 expression does appear to be associated with accumulation of infiltrating T lymphocytes and LC.
本研究探讨了口腔扁平苔藓（OLP）病变中上皮内白细胞是否存在选择性募集和分布的问题。使用流式细胞术和双重免疫荧光法检测了患者和对照组上皮及外周血中的T淋巴细胞亚群，并研究了角质形成细胞细胞间黏附分子-1（ICAM-1）表达与T淋巴细胞及朗格汉斯细胞（LC）分布之间的关系。辅助性T细胞（CD4+）的循环“记忆”亚群（CD45RO+）在对照组中占49.1%，在患者中增至65.7%（P=0.005），而对照组上皮中不存在的“初始”亚群（CD45RA+）在OLP中占辅助性T细胞的24%（P=0.016）。与对照组相比，OLP中表达CD45RO的LC较少（P=0.037），并且在上皮中表达ICAM-1的区域，所有T细胞和LC计数均显著升高。这些数据表明，与正常口腔黏膜相比，上皮内T淋巴细胞和LC群体发生了变化，并提示在OLP中存在选择性募集。此外，角质形成细胞ICAM-1表达似乎确实与浸润性T淋巴细胞和LC的聚集有关。
