Wada Y, Amiel M, Harwood F, Moriya H, Amiel D
Department of Orthopaedic Surgery, School of Medicine, Chiba University, Japan.
Arthroscopy. 1998 Apr;14(3):250-7. doi: 10.1016/s0749-8063(98)70140-9.
This study assesses the biological characteristics of cell repopulation and matrix remodeling in deep frozen meniscal allografts after total meniscectomy and allograft transplantation. Thirty-two mature New Zealand White rabbits were operated on, and the allografts were assessed at 12 and 26 weeks postsurgery. Before transplantation, fresh medial menisci were placed into liquid nitrogen to kill all intrinsic cells. Frozen meniscal allografts showed collagen remodeling coincident with revascularization and cellular repopulation. Biochemical characterization showed active collagen remodeling of the allografts at 12 and 26 weeks. In situ hybridization showed that expression of types I and III procollagen mRNAs were increased at 12 weeks, especially near the region of the synovial capsule. At 26 weeks after transplantation, the pattern of collagen-type expression progressed toward normal; that is, type I procollagen mRNA expression was prominent, whereas only a small amount of type III procollagen mRNA was observed. These data indicate the adaptation of the repopulating cells from the host to the frozen allograft at 26 weeks after transplantation.
本研究评估了全半月板切除和同种异体移植后深冻半月板同种异体移植物中细胞再填充和基质重塑的生物学特性。对32只成年新西兰白兔进行手术，并在术后12周和26周对同种异体移植物进行评估。移植前，将新鲜的内侧半月板放入液氮中以杀死所有固有细胞。冷冻半月板同种异体移植物显示出与血管再生和细胞再填充同时发生的胶原重塑。生化特征显示同种异体移植物在12周和26周时存在活跃的胶原重塑。原位杂交显示I型和III型前胶原mRNA在12周时表达增加，尤其是在滑膜囊区域附近。移植后26周，胶原类型表达模式向正常发展；即I型前胶原mRNA表达突出，而仅观察到少量III型前胶原mRNA。这些数据表明移植后26周宿主再填充细胞对冷冻同种异体移植物的适应性。
