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恶臭假单胞菌U中苯乙酸分解代谢途径的分子特征：苯乙酰辅酶A分解代谢单元

Molecular characterization of the phenylacetic acid catabolic pathway in Pseudomonas putida U: the phenylacetyl-CoA catabolon.

作者信息

Olivera E R, Miñambres B, García B, Muñiz C, Moreno M A, Ferrández A, Díaz E, García J L, Luengo J M

机构信息

Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad de León, 24007 León, Spain.

出版信息

Proc Natl Acad Sci U S A. 1998 May 26;95(11):6419-24. doi: 10.1073/pnas.95.11.6419.

DOI:10.1073/pnas.95.11.6419
PMID:9600981
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC27761/
Abstract

Fourteen different genes included in a DNA fragment of 18 kb are involved in the aerobic degradation of phenylacetic acid by Pseudomonas putida U. This catabolic pathway appears to be organized in three contiguous operons that contain the following functional units: (i) a transport system, (ii) a phenylacetic acid activating enzyme, (iii) a ring-hydroxylation complex, (iv) a ring-opening protein, (v) a beta-oxidation-like system, and (vi) two regulatory genes. This pathway constitutes the common part (core) of a complex functional unit (catabolon) integrated by several routes that catalyze the transformation of structurally related molecules into a common intermediate (phenylacetyl-CoA).

摘要

恶臭假单胞菌U中一个18 kb DNA片段所含的14种不同基因参与苯乙酸的需氧降解。这条分解代谢途径似乎由三个相邻的操纵子组成，这些操纵子包含以下功能单元：(i) 一个转运系统，(ii) 一种苯乙酸激活酶，(iii) 一个环羟基化复合体，(iv) 一种开环蛋白，(v) 一个类β-氧化系统，以及(vi) 两个调控基因。这条途径构成了一个复杂功能单元（分解代谢元）的共同部分（核心），该功能单元由几条途径整合而成，这些途径催化结构相关分子转化为一个共同中间体（苯乙酰辅酶A）。

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