Renner H, Renner K H, Hassenstein E
Strahlentherapie. 1976 Aug;152(2):140-8.
More than 300 lymphocyte cultures of 12 patients with seminomas were examined during the prophylactic radiotherapy and, in several cases, during an extended period until 20.5 months after the end of the treatment. The object of this study was to find out by measuring the capacity of the lymphocytes to be stimulated in vitro wheather they could be damaged by the radiotherapy. Among other reasons, the above mentioned patients were chosen because they had been submitted to irradiations of vast volumes of lymphatic tissues at a uniform focal dose of 4000 rad. The different opinions expressed in the literature (stimulation decreassed resp. increased resp. unchanged) are reflected by our results in such a way that we did not find a qualitative loss of the capacity to be stimulated cultures. The problem of the different opinions about the capacity of lymphocytes to be stimulated after a radiotherapy appears; among other things, to be based on different examination methods. According to these methods- morphological determination of the relative number of lymphoblasts, synthesis of DNA by fluid scintillation counting, or determination of the number of surviving cells in vitro -different results are obtained. It seems not possible to use the lymphocyte stimulation in vitro as a method of testing clinical sideefects occuring during the characteristics of immunity and radiation biology are not differentiated in a more precise manner.
在12例精原细胞瘤患者进行预防性放疗期间，对其300多个淋巴细胞培养物进行了检测，在某些情况下，检测时间延长至治疗结束后20.5个月。本研究的目的是通过测量淋巴细胞在体外被刺激的能力，来确定它们是否会受到放疗的损伤。选择上述患者的原因之一是，他们接受了4000拉德均匀局部剂量的大量淋巴组织照射。文献中表达的不同观点（刺激减少、增加或不变）在我们的结果中有所体现，即我们没有发现被刺激培养物的能力出现质性丧失。放疗后淋巴细胞被刺激能力的不同观点问题似乎，除其他外，基于不同的检测方法。根据这些方法——通过形态学确定淋巴母细胞的相对数量、通过液体闪烁计数法检测DNA合成或体外存活细胞数量的测定——会得到不同的结果。由于免疫特性和放射生物学特性没有更精确地区分，似乎不可能将体外淋巴细胞刺激作为一种检测临床副作用的方法。
