Güttes S, Failing K, Neumann K, Kleinstein J, Georgii S, Brunn H
Staatl. Medizinal-, Lebensmittel- und Veterinäruntersuchungsamt Mittelhessen, Marburger Str. 54, D-35396 Giessen, Germany.
Arch Environ Contam Toxicol. 1998 Jul;35(1):140-7. doi: 10.1007/s002449900361.
Persistent chlorinated hydrocarbons assimilated through the diet may, as a result of their carcinogenic, immunotoxic, and, at least in regard to certain of these substances, estrogenic properties, play a role in the etiology of human breast cancer. As a consequence, increased concentrations of these ubiquitous environmental contaminants may be found in breast tissue of women suffering from malignant breast disease. To examine this possibility, surgically removed breast tissue samples from 65 women in Hesse, Germany were examined by capillary gas chromatography for p, p'-dichloro(diphenyl)trichloroethane (p,p'-DDT), p, p'-dichloro(diphenyl)-dichloroethane (p,p'-DDD), p, p'-dichloro(diphenyl)dichloroethene (p,p'-DDE), hexachlorobenzine (HCB), alpha-, beta-, and gamma-hexachlorocyclohexane (HCH) as well as the polychlorinated biphenyls (PCB) no. 28, 31, 49, 52, 101, 105, 118, 138, 153, 156, 170, and 180. Of the 65 patients, 45 were diagnosed with breast cancer. The control group of 20 women suffered from benign breast disease such as mastopathy. After statistical adjustment for age differences, higher concentrations of p,p'-DDT, p, p'-DDE, HCB as well as PCB-congeners no. 118, 138, 153, and 180 were detected in tissue from women with breast cancer than in tissue from control persons. These differences were weakly significant for p, p'-DDE (p = 0.017), for PCB 118 (p = 0.042) and for PCB no. 153 barely not significant (p = 0.083). On an average, a 62% higher concentration of p,p'-DDE was found in cancer tissue (cancer patients: 805 microg/kg fat; controls: 496 microg/kg fat) and 25% higher concentration of PCB no. 118 (81 microg/kg fat; 65 microg/kg fat). The concentrations of beta-HCH, PCB no. 156 and 170 were lower (not significant) in cancer tissue than in tissue from women with benign disease. PCB-congeners no. 105 and 149 as well as gamma-HCH could only be detected in individual tissue samples; congeners no. 28, 31, 49, 52, and 101 as well as alpha-HCH and p,p'-DDD were not detected in any of the samples. To rule out the possibility that the concentrations of chlorinated hydrocarbons measured were influenced by the surgical procedure, 20 samples of tissue that were at a distance (minimum 1 cm and maximum 3 cm) from the tumor, tissue that was in direct proximity to the tumor (no more than 5 mm from the tumor), and tumor tissue itself (center of tumor) were separately prepared and analyzed. The average concentrations of chlorinated hydrocarbons varied to differing degrees and only minimally in tumor and surrounding breast tissue, indicating that the surgical procedure did not influence the results.
通过饮食摄入的持久性氯代烃，由于其致癌、免疫毒性以及（至少就其中某些物质而言）雌激素特性，可能在人类乳腺癌的病因中发挥作用。因此，在患有恶性乳腺疾病的女性乳腺组织中可能会发现这些普遍存在的环境污染物浓度升高。为了检验这种可能性，对德国黑森州65名女性手术切除的乳腺组织样本进行了毛细管气相色谱分析，检测其中的对，对'-二氯（二苯基）三氯乙烷（p,p'-滴滴涕）、对，对'-二氯（二苯基）二氯乙烷（p,p'-滴滴滴）、对，对'-二氯（二苯基）二氯乙烯（p,p'-滴滴伊）、六氯苯（HCB）、α-、β-和γ-六氯环己烷（HCH）以及多氯联苯（PCB）28、31、49、52、101、105、118、138、153、156、170和180。65名患者中，45名被诊断患有乳腺癌。20名女性的对照组患有乳腺病等良性乳腺疾病。在对年龄差异进行统计调整后，与对照组相比，乳腺癌患者组织中检测到的p,p'-滴滴涕、p,p'-滴滴伊、六氯苯以及PCB同系物118、138、153和180的浓度更高。这些差异中，p,p'-滴滴伊（p = 0.017）、PCB 118（p = 0.042）以及PCB 153（p = 0.083）勉强未达显著水平。平均而言，癌组织中p,p'-滴滴伊的浓度高出62%（癌症患者：805微克/千克脂肪；对照组：496微克/千克脂肪），PCB 118的浓度高出25%（81微克/千克脂肪；65微克/千克脂肪）。癌组织中β-六氯环己烷、PCB 156和170的浓度低于（不显著）患有良性疾病女性的组织。PCB同系物105和149以及γ-六氯环己烷仅在个别组织样本中检测到；同系物28、31、49、52和101以及α-六氯环己烷和p,p'-滴滴滴在任何样本中均未检测到。为排除所测氯代烃浓度受手术操作影响的可能性，分别制备并分析了距离肿瘤（最小1厘米，最大3厘米）、紧邻肿瘤（距肿瘤不超过5毫米）的组织以及肿瘤组织本身（肿瘤中心）的20个组织样本。氯代烃的平均浓度在不同程度上有所变化，在肿瘤和周围乳腺组织中变化极小，表明手术操作未影响结果。
