Kokunin V A, Delevaia V P, Pronina Z V, Gulyĭ M F
Ukr Biokhim Zh. 1976 Apr-Jun;48(3):348-54.
Carboxylation intensity was studied in the liver of young and adult cocks, 14C-bi-carbonate was used as an indicator of the amino acids, proteins and lipids turnover. It is established that the amount of dry residue in the liver tissue of the adult cocks is 22% higher and that of pyruvate and dicarboxylic amino acids is twice as low. The relative weight of the liver changes with age: in the 18-month-old cocks it is 1.5%, in the one-month-old cocks, 2.75%. The maximum of 14C incorporation into the tissue, dicarboxylic compounds, proteins and lipids of young cocks in the in vivo experiments is at a shorter exposure of the isotope. 14C removal from these compounds in the young cocks occurs more quickly as well. A higher radioactivity of the compounds under study in the adult cocks liver with prolongation of the isotope exposure is due to the effect of factors which are not related to the synthesis. The experiments established that radioactivity of proteins and lipids in the young cock liver slices is 6 times as high at 60-minute exposure. The mentioned above evidences for a higher rate of carboxylation in young cocks as compared to adult ones. When interpreting the data of metabolism study by means of isotopes in animals of different age a corrections should be made for a change in the liver relative weight during the growth process.
研究了幼年和成年公鸡肝脏中的羧化强度，使用14C-碳酸氢盐作为氨基酸、蛋白质和脂质周转的指标。结果表明，成年公鸡肝脏组织中的干残渣量高22%，而丙酮酸和二羧酸氨基酸的量则低两倍。肝脏的相对重量随年龄变化：18个月大的公鸡为1.5%，1个月大的公鸡为2.75%。在体内实验中，幼年公鸡组织、二羧酸化合物、蛋白质和脂质中14C掺入的最大值出现在同位素暴露时间较短时。幼年公鸡体内这些化合物中14C的去除也更快。随着同位素暴露时间的延长，成年公鸡肝脏中所研究化合物的放射性较高，这是由于与合成无关的因素的影响。实验表明，在60分钟的暴露时间下，幼年公鸡肝脏切片中蛋白质和脂质的放射性高6倍。上述结果表明，幼年公鸡的羧化速率高于成年公鸡。在解释不同年龄动物同位素代谢研究数据时，应针对生长过程中肝脏相对重量的变化进行校正。
