膦甲酸对疱疹病毒群的选择性抑制作用：对病毒DNA合成及病毒诱导的DNA聚合酶活性的影响。
Preferential inhibition of herpes-group viruses by phosphonoacetic acid: effect on virus DNA synthesis and virus-induced DNA polymerase activity.
作者信息
Huang E S, Huang C H, Huong S M, Selgrade M
出版信息
Yale J Biol Med. 1976 Mar;49(1):93-9.
Abstract
In tissue culture phosphonoacetic acid (PAA) specifically inhibited DNA synthesis of human cytomegalovirus (CMV), murine CMV, simian CMV, Epstein-Barr virus, and Herpesvirus saimiri. Fifty to one hundred micrograms per milliliter PAA completely inhibited viral DNA synthesis with no significant damage to host cell DNA synthesis. In vitro DNA polymerization assays showed that 10 μg/ml of PAA specifically inhibited partially purified human CMV-induced DNA polymerase, while little inhibition of host-cell DNA polymerase activity was found. The specific inhibition of herpes-group virus DNA synthesis with little toxicity to host cells suggests that PAA has great potential as an antiherpesvirus therapeutic agent.
摘要
在组织培养中，膦甲酸钠（PAA）特异性抑制人巨细胞病毒（CMV）、鼠巨细胞病毒、猿猴巨细胞病毒、爱泼斯坦-巴尔病毒和赛米利疱疹病毒的DNA合成。每毫升50至100微克的PAA可完全抑制病毒DNA合成，而对宿主细胞DNA合成无明显损害。体外DNA聚合酶测定表明，10微克/毫升的PAA可特异性抑制部分纯化的人CMV诱导的DNA聚合酶，而对宿主细胞DNA聚合酶活性的抑制作用很小。对疱疹病毒组病毒DNA合成的特异性抑制且对宿主细胞毒性较小，表明PAA作为抗疱疹病毒治疗剂具有很大潜力。
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