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用于检测呼肠孤样病毒的免疫电渗电泳:方法及与电子显微镜检查的比较

Immunoelectroosmophoresis for detection of reo-like virus: methodology and comparison with electron microscopy.

作者信息

Tufvesson B, Johnsson T

出版信息

Acta Pathol Microbiol Scand B. 1976 Aug;84(4):225-8. doi: 10.1111/j.1699-0463.1976.tb01929.x.

DOI:10.1111/j.1699-0463.1976.tb01929.x
PMID:961433
Abstract

In two separate studies, faecal samples were collected from children with acute gastroenteritis; the samples were tested both by electron microscopy and immunoelectroosmophoresis with a view to detect reo-like virus. In 94 per cent and 100 per cent of samples positive by electron microscopy in the first and second material, respectively, a precipitin line was found after electrophoresis, using a guinea pig antiserum against a purified bovine virus. No false positives were detected by this method.

摘要

在两项独立研究中,从患有急性肠胃炎的儿童身上采集了粪便样本;对样本进行了电子显微镜检查和免疫电渗电泳,以检测类呼肠孤病毒。在第一项和第二项材料中,分别有94%和100%经电子显微镜检查呈阳性的样本,在使用豚鼠抗纯化牛病毒血清进行电泳后发现了沉淀线。该方法未检测到假阳性。

相似文献

1
Immunoelectroosmophoresis for detection of reo-like virus: methodology and comparison with electron microscopy.用于检测呼肠孤样病毒的免疫电渗电泳:方法及与电子显微镜检查的比较
Acta Pathol Microbiol Scand B. 1976 Aug;84(4):225-8. doi: 10.1111/j.1699-0463.1976.tb01929.x.
2
Family infections by reo-like virus. Comparison of antibody titres by complement fixation and immunoelectroosmophoresis.类呼肠孤病毒引起的家庭感染。通过补体结合试验和免疫电渗电泳法比较抗体滴度。
Scand J Infect Dis. 1977;9(4):257-61. doi: 10.3109/inf.1977.9.issue-4.01.
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Rapid diagnosis of rotavirus infections: comparison of electron microscopy and immunoelectroosmophoresis for the detection of rotavirus in human infantile gastroenteritis.轮状病毒感染的快速诊断:电子显微镜检查与免疫电渗电泳法在检测人类婴幼儿肠胃炎中轮状病毒的比较
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Occurrence of reo-like calf viruses in young children with acute gastroenteritis. Diagnoses established by electron microscopy and complement fixation, using the reo-like virus as antigen.急性胃肠炎幼儿中类呼肠孤病毒的出现。通过电子显微镜检查和补体结合试验确诊,以类呼肠孤病毒作为抗原。
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Comparison of solid-phase immune electron microscopy, direct electron microscopy and enzyme-linked immunosorbent assay for detection of rotavirus in faecal samples.固相免疫电子显微镜、直接电子显微镜和酶联免疫吸附测定法检测粪便样本中轮状病毒的比较。
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Detection of a new virus by electron microscopy of faecal extracts from children with acute gastroenteritis.通过对急性肠胃炎患儿粪便提取物进行电子显微镜检查来检测一种新病毒。
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A reovirus-like agent (rotavirus) in gastroenteritis of children. Virus detection and serological studies.一种类似呼肠孤病毒的病原体(轮状病毒)与儿童肠胃炎。病毒检测及血清学研究。
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[Electronmicroscopic visualization of virusparticles in cases of gastroenteritis of infants and children (author's transl)].婴幼儿肠胃炎病例中病毒颗粒的电子显微镜观察(作者译)
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In vitro cultivation in human fetal intestinal organ culture of a reovirus-like agent associated with nonbacterial gastroenteritis in infants and children.在人胎儿肠道器官培养中对一种与婴幼儿非细菌性肠胃炎相关的呼肠孤病毒样病原体进行体外培养。
J Infect Dis. 1974 Nov;130(5):523-8. doi: 10.1093/infdis/130.5.523.

引用本文的文献

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Human viral gastroenteritis.人类病毒性肠胃炎
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Viral gastroenteritis.病毒性肠胃炎
Med Clin North Am. 1982 May;66(3):575-95. doi: 10.1016/s0025-7125(16)31408-0.
3
Seasonal fluctuations in the occurrence of enterotoxigenic bacteria and rotavirus in paediatric diarrhoea in Addis Ababa.亚的斯亚贝巴小儿腹泻中肠毒素产生菌和轮状病毒出现的季节性波动。
Bull World Health Organ. 1981;59(1):67-73.
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Human rotavirus antigen detection by enzyme-immunoassay with antisera against Nebraska calf diarrhoea virus.用抗内布拉斯加小牛腹泻病毒抗血清通过酶免疫测定法检测人轮状病毒抗原
J Clin Pathol. 1981 Jun;34(6):680-5. doi: 10.1136/jcp.34.6.680.
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Arch Virol. 1981;67(4):341-4. doi: 10.1007/BF01314837.
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Human viral gastroenteritis.人类病毒性肠胃炎
Clin Microbiol Rev. 1989 Jan;2(1):51-89. doi: 10.1128/CMR.2.1.51.
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Comparison of Counterimmunoelectrophoresis and electron microscopy for laboratory diagnosis of human reovirus-like agent-associated infantile gastroenteritis.对流免疫电泳与电子显微镜检查用于人类呼肠孤病毒样因子相关婴儿肠胃炎实验室诊断的比较
J Clin Microbiol. 1977 Feb;5(2):248-9. doi: 10.1128/jcm.5.2.248-249.1977.
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Infect Immun. 1979 Nov;26(2):686-9. doi: 10.1128/iai.26.2.686-689.1979.
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J Clin Pathol. 1979 Jul;32(7):700-5. doi: 10.1136/jcp.32.7.700.