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用于检测呼肠孤样病毒的免疫电渗电泳:方法及与电子显微镜检查的比较

Immunoelectroosmophoresis for detection of reo-like virus: methodology and comparison with electron microscopy.

作者信息

Tufvesson B, Johnsson T

出版信息

Acta Pathol Microbiol Scand B. 1976 Aug;84(4):225-8. doi: 10.1111/j.1699-0463.1976.tb01929.x.

Abstract

In two separate studies, faecal samples were collected from children with acute gastroenteritis; the samples were tested both by electron microscopy and immunoelectroosmophoresis with a view to detect reo-like virus. In 94 per cent and 100 per cent of samples positive by electron microscopy in the first and second material, respectively, a precipitin line was found after electrophoresis, using a guinea pig antiserum against a purified bovine virus. No false positives were detected by this method.

摘要

在两项独立研究中,从患有急性肠胃炎的儿童身上采集了粪便样本;对样本进行了电子显微镜检查和免疫电渗电泳,以检测类呼肠孤病毒。在第一项和第二项材料中,分别有94%和100%经电子显微镜检查呈阳性的样本,在使用豚鼠抗纯化牛病毒血清进行电泳后发现了沉淀线。该方法未检测到假阳性。

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