在日本从卵形硬蜱（蜱螨亚纲：硬蜱科）中分离出蜱传脑炎病毒。
Isolation of tick-borne encephalitis virus from Ixodes ovatus (Acari: Ixodidae) in Japan.
作者信息
Takeda T, Ito T, Chiba M, Takahashi K, Niioka T, Takashima I
机构信息
Department of Environmental Medicine Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Japan.
出版信息
J Med Entomol. 1998 May;35(3):227-31. doi: 10.1093/jmedent/35.3.227.
A case of tick-borne encephalitis (TBE) was found in a farming area located in the southern part of Hokkaido, Japan, in 1993. TBE viruses were isolated from sentinel dogs in the area where the human case occurred in 1995. Ticks were collected in the area by 2 collection methods in 1995 and virus isolation was conducted on ticks in 1995 and 1996. Ixodes ovatus Neumann was found to be the predominant tick species in the area. In 1996, 2 virus strains were isolated from 600 I. ovatus ticks (300 females and 300 males), giving the minimum infection rate of 0.33% (2 of 600). The 2 virus strains were identified as TBE virus by antigenic analysis using monoclonal antibodies and the indirect immunofluorescent antibody test. The results showed that I. ovatus was a suspected vector of the emerging TBE virus in Hokkaido.
1993年，在日本北海道南部的一个农业地区发现了一例蜱传脑炎（TBE）病例。1995年，在该人类病例发生地区的哨兵犬身上分离出了TBE病毒。1995年通过两种采集方法在该地区采集蜱虫，并于1995年和1996年对蜱虫进行病毒分离。发现卵形硬蜱是该地区的主要蜱种。1996年，从600只卵形硬蜱（300只雌蜱和300只雄蜱）中分离出2株病毒，最低感染率为0.33%（600只中的2只）。通过使用单克隆抗体的抗原分析和间接免疫荧光抗体试验，将这2株病毒鉴定为TBE病毒。结果表明，卵形硬蜱是北海道新出现的TBE病毒的疑似传播媒介。
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