Benjamin D R, Robinson C V, Hendrick J P, Hartl F U, Dobson C M
Oxford Centre for Molecular Sciences, New Chemistry Laboratory, University of Oxford, South Parks Road, Oxford OX1 3QT, United Kingdom.
Proc Natl Acad Sci U S A. 1998 Jun 23;95(13):7391-5. doi: 10.1073/pnas.95.13.7391.
Nanoflow electrospray ionization has been used to introduce intact Escherichia coli ribosomes into the ion source of a mass spectrometer. Mass spectra of remarkable quality result from a partial, but selective, dissociation of the particles within the mass spectrometer. Peaks in the spectra have been assigned to individual ribosomal proteins and to noncovalent complexes of up to five component proteins. The pattern of dissociation correlates strongly with predicted features of ribosomal protein-protein and protein-RNA interactions. The spectra allow the dynamics and state of folding of specific proteins to be investigated in the context of the intact ribosome. This study demonstrates a potentially general strategy to probe interactions within complex biological assemblies.
纳升电喷雾电离已被用于将完整的大肠杆菌核糖体引入质谱仪的离子源。质谱仪内粒子的部分但选择性解离产生了质量卓越的质谱图。光谱中的峰已被确定为单个核糖体蛋白以及多达五个组成蛋白的非共价复合物。解离模式与核糖体蛋白 - 蛋白和蛋白 - RNA相互作用的预测特征密切相关。这些光谱允许在完整核糖体的背景下研究特定蛋白质的折叠动力学和状态。这项研究展示了一种探测复杂生物组装体内相互作用的潜在通用策略。
