Boes J, Eriksen L, Nansen P
Danish Centre for Experimental Parasitology, Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Frederiksberg.
Vet Parasitol. 1998 Feb 28;75(2-3):181-90. doi: 10.1016/s0304-4017(97)00197-0.
The effect of anthelmintic treatment of pigs on the embryonation and infectivity of Ascaris suum eggs isolated from expelled worms was investigated. Four groups of two naturally infected pigs were dosed with albendazole, pyrantel pamoate, ivermectin or piperazine dihydrochloride, respectively. Following worm expulsion, the eggs were removed from the uteri of female worms and embryonated in sulphuric acid. The infectivity of the embryonated eggs was tested through mouse inoculation. Egg development appeared normal in cultures from worms of the piperazine. pyrantel and ivermectin treated groups. In the albendazole cultures, egg development was largely arrested at the one-cell stage (81%). Where development occurred, irregular cell division was observed and only 7% of the eggs in the culture developed into fullgrown larvae. Following mouse inoculation with 2500 embryonated eggs, significantly lower lung larval counts on day 8 post inoculation (p.i.) were observed for mice in the piperazine and pyrantel treated groups (P < 0.01) compared to untreated controls. The larvae that developed in the eggs from ivermectin and albendazole treated groups appeared fully infective for mice. It was concluded that ovicidal activity of albendazole in vivo inhibits subsequent A. suum egg development in vitro; albendazole is, therefore, not suitable to obtain worms for egg embryonation to produce experimental inoculums. The anthelmintic treatment of pigs with ivermectin had only a limited effect on both embryonation and infectivity of A. suum eggs isolated from expelled worms.
研究了对猪进行驱虫治疗对从排出的蠕虫中分离出的猪蛔虫卵的胚胎发育和感染性的影响。将四组每组两只自然感染的猪分别用阿苯达唑、噻嘧啶、伊维菌素或二盐酸哌嗪进行给药。蠕虫排出后，从雌虫子宫中取出虫卵，并在硫酸中进行胚胎发育。通过小鼠接种测试胚胎化虫卵的感染性。来自用哌嗪、噻嘧啶和伊维菌素治疗组蠕虫的培养物中，虫卵发育看起来正常。在阿苯达唑培养物中，虫卵发育在单细胞阶段大多停滞（81%）。在有发育的情况下，观察到细胞分裂不规则，培养物中只有7%的虫卵发育成成熟幼虫。在用2500个胚胎化虫卵接种小鼠后，与未治疗的对照组相比，哌嗪和噻嘧啶治疗组的小鼠在接种后第8天的肺幼虫计数显著降低（P<0.01）。来自伊维菌素和阿苯达唑治疗组虫卵中发育的幼虫对小鼠似乎具有完全的感染性。得出的结论是，阿苯达唑在体内的杀卵活性抑制了后续猪蛔虫卵在体外的发育；因此，阿苯达唑不适合用于获取用于胚胎发育以生产实验接种物的虫卵。用伊维菌素对猪进行驱虫治疗对从排出的蠕虫中分离出的猪蛔虫卵的胚胎发育和感染性都只有有限的影响。
