荧光原位杂交揭示的瓦登海沉积物微生物群落组成
Microbial Community Composition of Wadden Sea Sediments as Revealed by Fluorescence In Situ Hybridization.
作者信息
Llobet-Brossa E, Rosselló-Mora R, Amann R
机构信息
Max Planck Institut für Marine Mikrobiologie, 28359 Bremen, Germany.
出版信息
Appl Environ Microbiol. 1998 Jul 1;64(7):2691-6. doi: 10.1128/AEM.64.7.2691-2696.1998.
Abstract
The microbial community composition of Wadden Sea sediments of the German North Sea coast was investigated by in situ hybridization with group-specific fluorescently labeled, rRNA-targeted oligonucleotides. A large fraction (up to 73%) of the DAPI (4',6-diamidino-2-phenylindole)-stained cells hybridized with the bacterial probes. Nearly 45% of the total cells could be further identified as belonging to known phyla. Members of the Cytophaga-Flavobacterium cluster were most abundant in all layers, followed by the sulfate-reducing bacteria.
摘要
通过与群体特异性荧光标记的、靶向rRNA的寡核苷酸进行原位杂交，对德国北海沿岸瓦登海沉积物中的微生物群落组成进行了研究。用4',6-二脒基-2-苯基吲哚（DAPI）染色的细胞中有很大一部分（高达73%）与细菌探针杂交。总细胞数中近45%可进一步鉴定为属于已知的门。噬纤维菌-黄杆菌簇的成员在所有层中最为丰富，其次是硫酸盐还原菌。
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