Honda S, Fujioka T, Tokieda M, Satoh R, Nishizono A, Nasu M
Second Department of Internal Medicine, Oita Medical University, Japan.
Cancer Res. 1998 Oct 1;58(19):4255-9.
Helicobacter pylori is classified by IARC/WHO as a definite human gastric carcinogen, despite "inadequate experimental evidence." To obtain direct evidence concerning this relationship, we investigated the histopathological findings of gastric mucosa using a model of H. pylori infection in Mongolian gerbils. The animals were challenged p.o. with H. pylori ATCC-43504 and sacrificed at 6, 12, and 18 months after inoculation for histological examination. All inoculated animals were infected with H. pylori. Severe infiltration of the lamina propria by polymorphonuclear and mononuclear cells appeared in the lesser curvature of the antrum, with an increase in epithelial cell proliferation, and the infiltration extended to the body. Atrophic gastritis and focal intestinal metaplasia also appeared in the lesser curvature of the antral mucosa at 6 months after inoculation. Intestinal metaplasia became severe, with dysplasia, after that. At 18 months after H. pylori inoculation, two of five infected animals showed three well-differentiated gastric cancers. The uninfected control animals showed no abnormal findings throughout the entire observation period. Here, it was confirmed that H. pylori infection alone causes gastric cancer in an animal model.
幽门螺杆菌被国际癌症研究机构/世界卫生组织列为明确的人类胃癌致癌物，尽管“实验证据不足”。为了获得有关这种关系的直接证据，我们使用蒙古沙鼠幽门螺杆菌感染模型研究了胃黏膜的组织病理学发现。动物经口接种幽门螺杆菌ATCC - 43504，并在接种后6、12和18个月处死以进行组织学检查。所有接种动物均感染了幽门螺杆菌。在胃窦小弯处出现固有层被多形核细胞和单核细胞严重浸润，上皮细胞增殖增加，浸润扩展至胃体。接种后6个月，胃窦黏膜小弯处也出现萎缩性胃炎和灶性肠化生。此后，肠化生变得严重，并伴有发育异常。在幽门螺杆菌接种后18个月，五只感染动物中有两只出现了三个高分化胃癌。未感染的对照动物在整个观察期内未显示异常发现。在此，证实了仅幽门螺杆菌感染可在动物模型中导致胃癌。
