Ogden N H, Kurtenbach K, Nuttall P A
NERC Institute of Virology and Environmental Microbiology, Oxford, UK.
Exp Appl Acarol. 1998 Jun;22(6):367-72. doi: 10.1023/a:1024569226459.
The efficiency with which the spirochaete Borrelia burgdorferi sensu stricto was transmitted from laboratory mice to larval and nymphal Ixodes ricinus ticks was assessed, using the polymerase chain reaction. The transmission efficiency to nymphs was significantly greater than to larvae when both fed together on the same host. Increased tick infestation levels of mice were correlated with significantly greater engorgement weights and higher B. burgdorferi transmission coefficients from mice to nymphs. These observations indicate that both the feeding success of ticks and the transmission coefficients from host to tick may be influenced by the tick infestation level of an infected host. The infestation level and the relative numbers of each life stage of the tick are factors which should be considered in the design of transmission experiments.
利用聚合酶链反应评估了严格意义上的伯氏疏螺旋体从实验室小鼠传播至蓖麻硬蜱幼虫和若虫的效率。当幼虫和若虫在同一宿主上共同取食时，向若虫的传播效率显著高于向幼虫的传播效率。小鼠上蜱虫感染水平的增加与显著更高的饱血重量以及从小鼠到若虫的伯氏疏螺旋体传播系数相关。这些观察结果表明，蜱虫的取食成功率以及从宿主到蜱虫的传播系数可能都受感染宿主上蜱虫感染水平的影响。蜱虫的感染水平以及蜱虫各生命阶段的相对数量是在传播实验设计中应予以考虑的因素。
