溶液中光活性黄色蛋白在其光循环过程中的结构和动态变化。

Structural and dynamic changes of photoactive yellow protein during its photocycle in solution.

作者信息

Rubinstenn G, Vuister G W, Mulder F A, Düx P E, Boelens R, Hellingwerf K J, Kaptein R

机构信息

Bijovet Center for Biomolecular Research, Utrecht University, The Netherlands.

出版信息

Nat Struct Biol. 1998 Jul;5(7):568-70. doi: 10.1038/823.

DOI:10.1038/823

PMID:9665170

Abstract

Light irradiation of photoactive yellow protein (PYP) induces a photocycle, in which red-shifted (pR) and blue-shifted (pB) intermediates have been characterized. An NMR study of the long-lived pB intermediate now reveals that it exhibits a large degree of disorder and exists as a family of multiple conformers that exchange on a millisecond time scale. This shows that the behavior of PYP in solution is different from what has been observed in the crystalline state. Furthermore, differential refolding to ground state pG is observed, whereby the central beta-sheet and parts of the helical structure are formed first and the region around the chromophore at a later stage.

摘要

光照射光敏黄色蛋白（PYP）会引发一个光循环，其中已对红移（pR）和蓝移（pB）中间体进行了表征。一项对长寿命pB中间体的核磁共振研究现在表明，它表现出很大程度的无序性，并且以多个构象异构体家族的形式存在，这些构象异构体在毫秒时间尺度上进行交换。这表明PYP在溶液中的行为与在晶体状态下观察到的不同。此外，观察到向基态pG的差异重折叠，由此首先形成中央β-折叠和螺旋结构的部分，而发色团周围的区域则在稍后阶段形成。