Murosaki S, Yamamoto Y, Ito K, Inokuchi T, Kusaka H, Ikeda H, Yoshikai Y
Research & Development Section, Takeda Food Products, Ltd., Itami, Japan.
J Allergy Clin Immunol. 1998 Jul;102(1):57-64. doi: 10.1016/s0091-6749(98)70055-7.
Food allergy is caused by production of IgE against dietary antigen induced by T(H2) response. IL-12 inhibits T(H2) responses and strongly suppresses IgE production. We have recently established a murine model for IgE production with a predominant T(H2) response induced by feeding antigen.
We here show a suppressive effect of heat-killed Lactobacillus plantarum L-137, a potent inducer of IL-12, on IgE production against naturally fed antigen in a murine model.
The ability of L. plantarum L-137 to induce IL-12 production was examined in vitro and in vivo. DBA/2 mice were fed a casein diet and injected intraperitoneally with L. plantarum L-137 from the beginning of feeding or 2 weeks later. Recombinant mouse IL-12 was also injected 2 weeks after the start of feeding. Casein-specific IgE and IgG1 in plasma were determined by ELISA.
L. plantarum L-137 directly induced IL-12 production by the peritoneal macrophages and also stimulated spleen cells to produce both IL-12 and interferon-gamma in vitro. In vivo treatment of L. plantarum L-137 also increased the plasma level of IL-12 in mice. Plasma anti-casein IgG1 and IgE levels were gradually elevated in DBA/2 mice fed a casein diet. Administration of L. plantarum L-137 from the beginning of feeding suppressed the elevation of anti-casein IgE levels, whereas the levels of anti-casein IgG1 were rather augmented by L. plantarum L-137. IL-12 production of the peritoneal macrophages was enhanced, but IL-4 production of concanavalin A (Con A)-stimulated spleen cells was suppressed in the L. plantarum L-137-treated mice compared with control mice fed a casein diet. When L. plantarum L-137 was given from 2 weeks after the start of feeding, anti-casein IgE levels were also significantly suppressed, which was similar to the result found in mice treated with IL-12.
Our results suggest that L. plantarum L-137, a potent IL-12 inducer, is useful for prevention and treatment of food allergy.
食物过敏是由针对由T(H2)反应诱导的饮食抗原产生IgE引起的。白细胞介素-12抑制T(H2)反应并强烈抑制IgE产生。我们最近建立了一个通过喂食抗原诱导主要T(H2)反应的小鼠IgE产生模型。
我们在此展示了热灭活的植物乳杆菌L-137（一种白细胞介素-12的有效诱导剂）对小鼠模型中针对自然喂食抗原产生的IgE的抑制作用。
在体外和体内检测植物乳杆菌L-137诱导白细胞介素-12产生的能力。从喂食开始或2周后，给DBA/2小鼠喂食酪蛋白饮食并腹腔注射植物乳杆菌L-137。在喂食开始2周后也注射重组小鼠白细胞介素-12。通过酶联免疫吸附测定法测定血浆中酪蛋白特异性IgE和IgG1。
植物乳杆菌L-137直接诱导腹膜巨噬细胞产生白细胞介素-12，并且在体外还刺激脾细胞产生白细胞介素-12和干扰素-γ。植物乳杆菌L-137的体内处理也增加了小鼠血浆中白细胞介素-12的水平。喂食酪蛋白饮食的DBA/2小鼠血浆中抗酪蛋白IgG1和IgE水平逐渐升高。从喂食开始就给予植物乳杆菌L-137抑制了抗酪蛋白IgE水平的升高，而植物乳杆菌L-137使抗酪蛋白IgG1水平有所增加。与喂食酪蛋白饮食的对照小鼠相比，植物乳杆菌L-137处理的小鼠腹膜巨噬细胞的白细胞介素-12产生增强，但伴刀豆球蛋白A（Con A）刺激的脾细胞的白细胞介素-4产生受到抑制。当从喂食开始2周后给予植物乳杆菌L-137时,抗酪蛋白IgE水平也被显著抑制，这与用白细胞介素-12处理的小鼠的结果相似。
我们的结果表明，植物乳杆菌L-137这种有效的白细胞介素-12诱导剂，对食物过敏的预防和治疗有用。
