Baillie G S, Douglas L J
Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, United Kingdom.
Antimicrob Agents Chemother. 1998 Aug;42(8):2146-9. doi: 10.1128/AAC.42.8.2146.
Biofilms of Candida albicans were grown in vitro under iron limitation and at a low growth rate to simulate conditions for implant-associated biofilms in vivo. Their properties were compared with those of glucose-limited biofilms grown under analogous conditions. At steady state, the adherent cell populations of iron-limited biofilms were double those of glucose-limited biofilms, although the growth rates were similar (0.038 to 0.043 h-1). Both biofilm types were resistant to amphotericin B, but daughter cells from iron-limited biofilms were significantly more susceptible to the drug than those from glucose-limited biofilms.
白色念珠菌生物膜在体外铁限制和低生长速率条件下培养，以模拟体内植入相关生物膜的条件。将它们的特性与在类似条件下培养的葡萄糖限制生物膜的特性进行比较。在稳定状态下，铁限制生物膜的贴壁细胞数量是葡萄糖限制生物膜的两倍，尽管生长速率相似（0.038至0.043 h-1）。两种生物膜类型均对两性霉素B耐药，但铁限制生物膜的子细胞比葡萄糖限制生物膜的子细胞对该药物更敏感。
