Rabkin C S, Schulz T F, Whitby D, Lennette E T, Magpantay L I, Chatlynne L, Biggar R J
Viral Epidemiology Branch, National Cancer Institute, Bethesda, Maryland, USA.
J Infect Dis. 1998 Aug;178(2):304-9. doi: 10.1086/515649.
To standardize human herpesvirus 8 (HHV-8) antibody assays for application to asymptomatic infection, a blinded comparison was done of seven immunofluorescence assays and ELISAs. Five experienced laboratories tested a serum panel from 143 subjects in 4 diagnostic groups. Except for a minor capsid protein ELISA, the other six tests detected HHV-8 antibodies most frequently in classic (80%-100%) and AIDS-related (67%-91%) Kaposi's sarcoma, followed by human immunodeficiency virus-seropositive patients (27%-60%), and least frequently in healthy blood donors (0-29%). However, these six assays frequently disagreed on individual sera, particularly for blood donor samples. Current HHV-8 antibody tests have uncertain accuracy in asymptomatic HHV-8 infection and may require correlation with viral protein or nucleic acid detection. Antibody assays are useful for epidemiologic investigations, but the absolute prevalence of HHV-8 infection in the United States cannot yet be determined.
为使人类疱疹病毒8型（HHV-8）抗体检测标准化以应用于无症状感染，对7种免疫荧光检测法和酶联免疫吸附测定（ELISA）进行了盲法比较。5个经验丰富的实验室对来自4个诊断组的143名受试者的一组血清进行了检测。除一种次要衣壳蛋白ELISA外，其他6种检测在经典型（80%-100%）和艾滋病相关型（67%-91%）卡波西肉瘤患者中检测到HHV-8抗体的频率最高，其次是人类免疫缺陷病毒血清阳性患者（27%-60%），而在健康献血者中检测到的频率最低（0-29%）。然而，这6种检测方法对个体血清的检测结果常常不一致，尤其是对于献血者样本。目前的HHV-8抗体检测在无症状HHV-8感染中的准确性尚不确定，可能需要与病毒蛋白或核酸检测结果相互印证。抗体检测对于流行病学调查有用，但美国HHV-8感染的绝对流行率尚无法确定。
