通过聚合酶链反应（PCR）鉴别来自囊性纤维化患者中可检测到的其他伯克霍尔德菌属物种的唐菖蒲伯克霍尔德菌。
Discrimination of Burkholderia gladioli from other Burkholderia species detectable in cystic fibrosis patients by PCR.
作者信息
Bauernfeind A, Schneider I, Jungwirth R, Roller C
机构信息
Department of Experimental Medical Microbiology and Cystic Fibrosis, Max von Pettenkofer Institute, D-80336 Munich, Germany.
出版信息
J Clin Microbiol. 1998 Sep;36(9):2748-51. doi: 10.1128/JCM.36.9.2748-2751.1998.
Abstract
A procedure for molecular identification of Burkholderia gladioli is described. Specific 16S and 23S rRNA gene signature sequences were defined as primers for PCR. The method allows rapid and specific discrimination of B. gladioli from related species (B. cepacia, B. multivorans, B. vietnamiensis, B. mallei, B. pseudomallei, Ralstonia pickettii, and R. eutropha) and should contribute to the clarification of its role as a human pathogen, e.g., in cystic fibrosis.
摘要
描述了一种唐菖蒲伯克霍尔德菌分子鉴定的方法。特定的16S和23S rRNA基因特征序列被定义为PCR引物。该方法能够快速、特异性地将唐菖蒲伯克霍尔德菌与相关物种（洋葱伯克霍尔德菌、多食伯克霍尔德菌、越南伯克霍尔德菌、鼻疽伯克霍尔德菌、类鼻疽伯克霍尔德菌、皮氏罗尔斯顿菌和真养罗尔斯顿菌）区分开来，并且应该有助于阐明其作为人类病原体的作用，例如在囊性纤维化中的作用。
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