Vadiraja B B, Gaikwad N W, Madyastha K M
Department of Organic Chemistry, Indian Institute of Science, Bangalore, 560 012, India.
Biochem Biophys Res Commun. 1998 Aug 19;249(2):428-31. doi: 10.1006/bbrc.1998.9149.
Effect of C-phycocyanin (from Spirulina platensis) pretreatment on carbontetrachloride and R-(+)-pulegone-induced hepatotoxicity in rats was studied. Intraperitoneal (i.p.) administration (200 mg/kg) of a single dose of phycocyanin to rats, one or three hours prior to R-(+)-pulegone (250 mg/kg) or carbontetrachloride (0.6 ml/kg) challenge, significantly reduced the hepatotoxicity caused by these chemicals. For instance, serum glutamate pyruvate transaminase (SGPT) activity was almost equal to control values. The losses of microsomal cytochrome P450, glucose-6-phosphatase and aminopyrine-N-demethylase were significantly reduced, suggesting that phycocyanin provides protection to liver enzymes. It was noticed that the level of menthofuran, the proximate toxin of R-(+)-pulegone was nearly 70% more in the urine samples collected from rats treated with R-(+)-pulegone alone than rats treated with the combination of phycocyanin and R-(+)-pulegone. The possible mechanism involved in the hepatoprotection is discussed.
研究了来自钝顶螺旋藻的C-藻蓝蛋白预处理对四氯化碳和R-(+)-胡薄荷酮诱导的大鼠肝毒性的影响。在给予大鼠R-(+)-胡薄荷酮（250mg/kg）或四氯化碳（0.6ml/kg）攻击前1小时或3小时，腹腔注射（i.p.）单剂量的藻蓝蛋白（200mg/kg），可显著降低这些化学物质引起的肝毒性。例如，血清谷丙转氨酶（SGPT）活性几乎与对照值相等。微粒体细胞色素P450、葡萄糖-6-磷酸酶和氨基比林-N-脱甲基酶的损失显著减少，表明藻蓝蛋白对肝酶具有保护作用。值得注意的是，仅用R-(+)-胡薄荷酮处理的大鼠尿液样本中，R-(+)-胡薄荷酮的直接毒素薄荷呋喃水平比用藻蓝蛋白和R-(+)-胡薄荷酮联合处理的大鼠高出近70%。文中讨论了肝保护作用可能涉及的机制。
