Kalus W H, Münzner R, Filby W G
Federal Institute for Nutrition Research, Engesserstrasse 20, Karlsruhe, Germany.
Environ Health Perspect. 1994 Jan;102(1):96-9. doi: 10.1289/ehp.9410296.
We examined t-butylhydroquinone (t-BHQ) and t-butylquinone (t-BuQ), two of the major microsomal metabolites of the synthetic antioxidant butylated hydroxyanisole (BHA), for their ability to react with the xenobiotic arylamines aniline and N-methylaniline. A number of substances were isolated by thin-layer chromatography. The main products were quantitatively evaluated and their structures assigned. BHA and t-BHQ yielded reaction products with anilines only in the presence of an oxidant such as iodate (KIO3). We used the Salmonella/microsome mutagenicity assay to test the new compounds for mutagenic activity. The reaction products gave no evidence of mutagenicity in the S. typhimurium strains TA98 and TA100, with or without metabolic activation. In some instances the substituted quinone products are less toxic than t-BuQ alone.
我们研究了叔丁基对苯二酚（t-BHQ）和叔丁基醌（t-BuQ），它们是合成抗氧化剂丁基羟基茴香醚（BHA）的两种主要微粒体代谢产物，考察了它们与外源性芳胺苯胺和N-甲基苯胺发生反应的能力。通过薄层色谱法分离出了多种物质。对主要产物进行了定量评估并确定了其结构。BHA和t-BHQ仅在存在诸如碘酸盐（KIO3）等氧化剂的情况下才与苯胺生成反应产物。我们采用沙门氏菌/微粒体诱变性试验来检测这些新化合物的诱变活性。无论有无代谢活化，反应产物在鼠伤寒沙门氏菌TA98和TA100菌株中均未显示出诱变活性。在某些情况下，取代醌产物的毒性低于单独的t-BuQ。
