用新发现的猪戊型肝炎病毒（猪HEV）而非人源HEV毒株对猪进行实验性感染。

Experimental infection of pigs with the newly identified swine hepatitis E virus (swine HEV), but not with human strains of HEV.

作者信息

Meng X J, Halbur P G, Haynes J S, Tsareva T S, Bruna J D, Royer R L, Purcell R H, Emerson S U

机构信息

Hepatitis Viruses Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.

出版信息

Arch Virol. 1998;143(7):1405-15. doi: 10.1007/s007050050384.

DOI:10.1007/s007050050384

PMID:9722883

Abstract

A novel virus of pigs, swine hepatitis E virus (swine HEV), was recently identified and shown to be antigenically and genetically related to human HEV. In the present study, we attempted to infect specific-pathogen-free (SPF) pigs experimentally with swine HEV or with human strains of HEV. Serum samples collected from naturally infected pigs were used as the source of swine HEV. Pigs inoculated intravenously with serum samples containing swine HEV seroconverted to anti-HEV 4 to 8 weeks postinoculation, and the virus spread to an uninoculated pig. Swine HEV was detected in nasal and rectal swab materials as early as 2 weeks postinoculation and for 4 to 8 weeks thereafter. Viremia appeared 4 to 6 weeks postinoculation and lasted 1 to 3 weeks. The inoculated pigs appeared clinically normal and serum liver enzymes were not significantly elevated. In contrast, pigs were not infected when inoculated intravenously with about 10(5) monkey infectious doses of one of two human strains of HEV (Sar-55 or Mex-14).

摘要

一种新型猪病毒——猪戊型肝炎病毒（swine HEV），最近被鉴定出来，并显示出在抗原性和基因上与人类戊型肝炎病毒（HEV）相关。在本研究中，我们试图用猪戊型肝炎病毒或人类戊型肝炎病毒株对无特定病原体（SPF）猪进行实验性感染。从自然感染猪采集的血清样本用作猪戊型肝炎病毒的来源。静脉接种含有猪戊型肝炎病毒血清样本的猪在接种后4至8周血清转化为抗戊型肝炎病毒，并且病毒传播到未接种的猪。接种后2周最早在鼻拭子和直肠拭子样本中检测到猪戊型肝炎病毒，此后4至8周均可检测到。接种后4至6周出现病毒血症，持续1至3周。接种的猪临床外观正常，血清肝酶未显著升高。相比之下，静脉接种约10⁵个猴感染剂量的两种人类戊型肝炎病毒株之一（Sar-55或Mex-14）时，猪未被感染。