Schifter M, Jones A M, Walker D M
Department of Oral Diagnostic Sciences, Dental Clinical School, Westmead Hospital, NSW, Australia.
J Oral Pathol Med. 1998 Aug;27(7):318-24. doi: 10.1111/j.1600-0714.1998.tb01963.x.
The immunohistochemical detection of epithelial p53 protein expression in oral lichen planus (OLP) biopsies was supplemented with molecular analysis for mutations of the p53 gene using the polymerase chain reaction - single stranded conformational polymorphism (PCR-SSCP) technique. p53 protein expression, in the basal epithelial cell layer, as detected by the DO7 and 1801 antibodies, was significantly more frequent in OLP compared with other oral keratoses and normal mucosa, as was the growth fraction. The 10 OLP biopsies that had the most frequent p53 staining (plus a case of OLP found in continuity with a SCC) were screened by the PCR-SSCP technique, but no mutations were detected in the p53 gene (exons 5 9). The p53 overexpression in the OLP samples may be a physiological response to the hyper-proliferative state, as revealed by the growth fraction determination. This may usefully serve to protect against mutagenesis, and so be a factor in the low incidence of carcinoma associated with OLP.
通过聚合酶链反应-单链构象多态性（PCR-SSCP）技术对口腔扁平苔藓（OLP）活检组织中上皮p53蛋白表达进行免疫组化检测，并对p53基因的突变进行分子分析。DO7和1801抗体检测发现，OLP患者基底上皮细胞层中的p53蛋白表达及生长分数与其他口腔角化病和正常黏膜相比显著更常见。通过PCR-SSCP技术对10例p53染色最频繁的OLP活检组织（加上1例与鳞状细胞癌连续的OLP病例）进行筛查，但未在p53基因（外显子5至9）中检测到突变。生长分数测定显示，OLP样本中的p53过表达可能是对增殖状态的一种生理反应。这可能有助于防止诱变，因此是OLP相关癌发病率低的一个因素。
