Hauser B A, Villanueva J M, Gasser C S
Section of Molecular and Cellular Biology, Division of Biological Sciences, University of California, Davis, California 95616, USA.
Genetics. 1998 Sep;150(1):411-23. doi: 10.1093/genetics/150.1.411.
Flowers of the previously described Arabidopsis tso1-1 mutant had aberrant, highly reduced organs in place of petals, stamens, and carpels. Cells of tso1-1 flowers had division defects, including failure in cytokinesis, partial cell wall formation, and elevated nuclear DNA content. We describe here two new tso1 alleles (tso1-3 and tso1-4), which caused defects in ovule development, but had little effect on gross floral morphology. Early ovule development occurred normally in tso1-3 and tso1-4, but the shapes and alignments of integument cells became increasingly more disordered as development progressed. tso1-3 ovules usually lacked embryo sacs due to a failure to form megaspore mother cells. The cell division defects described for the strong tso1-1 mutant were rarely observed in tso1-3 ovules. The aberrations in tso1-3 mutants primarily resulted from a failure in directional expansion of cells and/or coordination of this process among adjacent cells. Effects of tso1-3 appeared to be independent of effects of other ovule development mutations, with the exception of leunig, which exhibited a synergistic interaction. The data are consistent with TSO1 acting in processes governing directional movement of cellular components, indicating a likely role for TSO1 in cytoskeletal function.
先前描述的拟南芥tso1-1突变体的花具有异常的、高度退化的器官，取代了花瓣、雄蕊和心皮。tso1-1花的细胞具有分裂缺陷，包括胞质分裂失败、部分细胞壁形成以及核DNA含量升高。我们在此描述两个新的tso1等位基因（tso1-3和tso1-4），它们导致胚珠发育缺陷，但对花的总体形态影响很小。tso1-3和tso1-4的早期胚珠发育正常，但随着发育的进行，珠被细胞的形状和排列变得越来越无序。tso1-3胚珠通常由于未能形成大孢子母细胞而缺乏胚囊。在tso1-3胚珠中很少观察到强tso1-1突变体所描述的细胞分裂缺陷。tso1-3突变体中的畸变主要是由于细胞定向扩展失败和/或相邻细胞之间这一过程的协调失败。tso1-3的影响似乎独立于其他胚珠发育突变的影响，除了与leunig表现出协同相互作用外。这些数据与TSO1在控制细胞成分定向运动的过程中起作用一致，表明TSO1在细胞骨架功能中可能发挥作用。
