脂质特性对跨膜蛋白结构的影响。
Effect of lipid characteristics on the structure of transmembrane proteins.
作者信息
Dan N, Safran S A
机构信息
Department of Chemical Engineering, University of Delaware, Newark 19716, USA.
出版信息
Biophys J. 1998 Sep;75(3):1410-4. doi: 10.1016/S0006-3495(98)74059-7.
Abstract
The activity of embedded proteins is known to vary with lipid characteristics. Indeed, it has been shown that some cell-membrane proteins cannot function unless certain non-bilayer-forming lipids (i.e., nonzero spontaneous curvature) are present. In this paper we show that membranes exert a line tension on transmembrane proteins. The line tension, on the order of 1-100 kT/protein, varies with the lipid properties and the protein configuration. Thus, membranes composed of different lipids favor different protein conformations. Model predictions are in excellent agreement with the data of Keller et al. (Biophys. J. 1993, 65:23-27) regarding the conductance of alamethicin channels.
摘要
已知嵌入蛋白质的活性会随脂质特性而变化。事实上，已经表明某些细胞膜蛋白只有在存在某些非双层形成脂质（即非零自发曲率）时才能发挥功能。在本文中，我们表明膜会对跨膜蛋白施加线张力。线张力的量级为1 - 100 kT/蛋白，它会随脂质性质和蛋白质构象而变化。因此，由不同脂质组成的膜有利于不同的蛋白质构象。模型预测与凯勒等人（《生物物理杂志》，1993年，65卷：23 - 27页）关于短杆菌肽通道电导的数据高度吻合。
相似文献
1
Effect of lipid characteristics on the structure of transmembrane proteins.脂质特性对跨膜蛋白结构的影响。
Biophys J. 1998 Sep;75(3):1410-4. doi: 10.1016/S0006-3495(98)74059-7.
2
Probability of alamethicin conductance states varies with nonlamellar tendency of bilayer phospholipids.短杆菌肽A电导状态的概率随双层磷脂的非层状倾向而变化。
Biophys J. 1993 Jul;65(1):23-7. doi: 10.1016/S0006-3495(93)81040-3.
3
An alamethicin channel in a lipid bilayer: molecular dynamics simulations.脂质双分子层中的短杆菌肽通道：分子动力学模拟
Biophys J. 1999 Apr;76(4):1757-69. doi: 10.1016/s0006-3495(99)77337-6.
4
Implicit solvent model estimates of the stability of model structures of the alamethicin channel.阿拉米辛通道模型结构稳定性的隐式溶剂模型估计
Eur Biophys J. 2004 Feb;33(1):16-28. doi: 10.1007/s00249-003-0345-4. Epub 2003 Sep 17.
5
Calculations suggest a pathway for the transverse diffusion of a hydrophobic peptide across a lipid bilayer.计算结果表明了一种疏水性肽在脂质双分子层中横向扩散的途径。
Biophys J. 2000 Nov;79(5):2322-30. doi: 10.1016/S0006-3495(00)76478-2.
6
Alamethicin channel conductance modified by lipid charge.被脂质电荷修饰的短杆菌肽通道电导
Eur Biophys J. 2001 Aug;30(4):233-41. doi: 10.1007/s002490100145.
7
The barrel-stave model as applied to alamethicin and its analogs reevaluated.应用于短杆菌肽及其类似物的桶板模型重新评估。
Biophys J. 1994 Feb;66(2 Pt 1):355-9. doi: 10.1016/s0006-3495(94)80784-2.
8
Surface binding of alamethicin stabilizes its helical structure: molecular dynamics simulations.阿拉米辛的表面结合稳定了其螺旋结构：分子动力学模拟
Biophys J. 1999 Jun;76(6):3186-91. doi: 10.1016/S0006-3495(99)77470-9.
9
Alpha-helical hydrophobic polypeptides form proton-selective channels in lipid bilayers.α-螺旋疏水多肽在脂质双层中形成质子选择性通道。
Biophys J. 1994 May;66(5):1364-79. doi: 10.1016/S0006-3495(94)80927-0.
10
Alamethicin helices in a bilayer and in solution: molecular dynamics simulations.双层膜中和溶液中的短杆菌肽螺旋：分子动力学模拟
Biophys J. 1999 Jan;76(1 Pt 1):40-9. doi: 10.1016/S0006-3495(99)77176-6.
引用本文的文献
1
Lipid liquid-crystalline nanoparticles as a suitable platform for accommodating sensitive membrane proteins: monitoring the activity of HMG-CoA reductase.脂质液晶纳米颗粒作为容纳敏感膜蛋白的合适平台：监测HMG-CoA还原酶的活性
J Nanobiotechnology. 2025 May 7;23(1):334. doi: 10.1186/s12951-025-03370-6.
2
Screening for bilayer-active and likely cytotoxic molecules reveals bilayer-mediated regulation of cell function.筛选双层活性和可能具有细胞毒性的分子揭示了双层介导的细胞功能调节。
J Gen Physiol. 2023 Apr 3;155(4). doi: 10.1085/jgp.202213247. Epub 2023 Feb 10.
3
Extent of raft composition in a model plasma membrane.模型质膜中筏的组成范围。
Biophys J. 2023 Jun 6;122(11):1956-1961. doi: 10.1016/j.bpj.2022.08.041. Epub 2022 Sep 1.
4
Lipid redistribution in the highly curved footprint of Piezo1.Piezo1 高度弯曲的足迹中的脂质再分布。
Biophys J. 2023 Jun 6;122(11):1900-1913. doi: 10.1016/j.bpj.2022.07.022. Epub 2022 Aug 4.
5
On the Role of Curved Membrane Nanodomains, and Passive and Active Skeleton Forces in the Determination of Cell Shape and Membrane Budding.在细胞形状和膜出芽的决定中，弯曲膜纳米域、被动和主动骨架力的作用。
Int J Mol Sci. 2021 Feb 26;22(5):2348. doi: 10.3390/ijms22052348.
6
Recent Experiments Support a Microemulsion Origin of Plasma Membrane Domains: Dependence of Domain Size on Physical Parameters.近期实验支持质膜结构域的微乳液起源：结构域大小对物理参数的依赖性。
Membranes (Basel). 2020 Jul 28;10(8):167. doi: 10.3390/membranes10080167.
7
Model Plasma Membrane Exhibits a Microemulsion in Both Leaves Providing a Foundation for "Rafts".模型质膜在两种叶片中均表现出微乳液，为“筏”提供了基础。
Biophys J. 2020 Mar 10;118(5):1019-1031. doi: 10.1016/j.bpj.2020.01.004. Epub 2020 Jan 16.
8
Functional Reconstitution of HlyB, a Type I Secretion ABC Transporter, in Saposin-A Nanoparticles.在 Saposin-A 纳米颗粒中功能性重建 HlyB，一种 I 型分泌 ABC 转运蛋白。
Sci Rep. 2019 Jun 10;9(1):8436. doi: 10.1038/s41598-019-44812-0.
9
Global small-angle scattering data analysis of inverted hexagonal phases.反六角相的全球小角散射数据分析
J Appl Crystallogr. 2019 Mar 28;52(Pt 2):403-414. doi: 10.1107/S1600576719002760. eCollection 2019 Apr 1.
10
The role of traction in membrane curvature generation.牵引在膜曲率生成中的作用。
Mol Biol Cell. 2018 Aug 8;29(16):2024-2035. doi: 10.1091/mbc.E18-02-0087. Epub 2018 Jul 25.
本文引用的文献
1
Entropy-driven tension and bending elasticity in condensed-fluid membranes.凝聚流体膜中的熵驱动张力和弯曲弹性。
Phys Rev Lett. 1990 Apr 23;64(17):2094-2097. doi: 10.1103/PhysRevLett.64.2094.
2
Gramicidin channel kinetics under tension.受张力作用下短杆菌肽通道的动力学
Biophys J. 1998 Jan;74(1):328-37. doi: 10.1016/S0006-3495(98)77790-2.
3
Lipid bilayer electrostatic energy, curvature stress, and assembly of gramicidin channels.脂质双分子层静电能、曲率应力与短杆菌肽通道组装
Biochemistry. 1997 May 13;36(19):5695-701. doi: 10.1021/bi9619841.
4
Biomembranes. Lipids beyond the bilayer.生物膜。双层膜之外的脂质。
Nature. 1997 Mar 13;386(6621):129-30. doi: 10.1038/386129a0.
5
The lateral pressure profile in membranes: a physical mechanism of general anesthesia.膜中的侧向压力分布：全身麻醉的一种物理机制。
Biochemistry. 1997 Mar 4;36(9):2339-44. doi: 10.1021/bi9627323.
6
Interaction between inclusions embedded in membranes.嵌入膜中的内含物之间的相互作用。
Biophys J. 1996 Aug;71(2):648-56. doi: 10.1016/S0006-3495(96)79265-2.
7
A phospholipid acts as a chaperone in assembly of a membrane transport protein.磷脂在膜转运蛋白的组装过程中起到伴侣蛋白的作用。
J Biol Chem. 1996 May 17;271(20):11615-8. doi: 10.1074/jbc.271.20.11615.
8
Non-bilayer lipids are required for efficient protein transport across the plasma membrane of Escherichia coli.非双层脂质是大肠杆菌蛋白质有效跨质膜转运所必需的。
EMBO J. 1995 Nov 15;14(22):5506-13. doi: 10.1002/j.1460-2075.1995.tb00237.x.
9
Probability of alamethicin conductance states varies with nonlamellar tendency of bilayer phospholipids.短杆菌肽A电导状态的概率随双层磷脂的非层状倾向而变化。
Biophys J. 1993 Jul;65(1):23-7. doi: 10.1016/S0006-3495(93)81040-3.
10
Intrinsic curvature hypothesis for biomembrane lipid composition: a role for nonbilayer lipids.生物膜脂质组成的内在曲率假说：非双层脂质的作用。
Proc Natl Acad Sci U S A. 1985 Jun;82(11):3665-9. doi: 10.1073/pnas.82.11.3665.
Zotero 插件