Peek R, Verbraak F, Coevoet H M, Kijlstra A
Netherlands Ophthalmic Research Institute, Amsterdam.
Invest Ophthalmol Vis Sci. 1998 Sep;39(10):1976-9.
To study the specificity of circulating retinal autoantibodies in a patient with progressive loss of vision resembling cancer-associated retinopathy in the absence of systemic malignancy.
Patient's serum was tested for the presence of antiretinal antibodies by Western blot analysis, and immunohistochemical analysis was performed on cryosections of rat retina and on cultured rat retinal Müller cells.
Western blot analysis revealed that the serum contained a high titer of autoantibodies against a 35-kDa retinal antigen. A protein of similar molecular weight and antigenicity has been found to be present in protein extracts of bovine, rat, and fish retina. Immunohistochemical analysis suggested that the autoantibodies did not localize to retinal neural cells, as reported for several other putative autoimmune retinal disorders, but rather to the retinal Müller cells. This cell type-specificity could be confirmed using purified cultured retinal Müller cells.
These results suggest that an autoimmune response directed against the retinal Müller cells, in the absence of overt systemic malignancy, may lead to a slow-developing visual deterioration.
研究在无全身恶性肿瘤情况下，一名视力逐渐丧失且类似癌症相关性视网膜病变患者循环视网膜自身抗体的特异性。
通过蛋白质印迹分析检测患者血清中抗视网膜抗体的存在情况，并对大鼠视网膜冰冻切片和培养的大鼠视网膜穆勒细胞进行免疫组织化学分析。
蛋白质印迹分析显示，血清中含有高滴度针对一种35 kDa视网膜抗原的自身抗体。已发现牛、大鼠和鱼视网膜的蛋白质提取物中存在一种分子量和抗原性相似的蛋白质。免疫组织化学分析表明，与其他几种假定的自身免疫性视网膜疾病不同，这些自身抗体并不定位于视网膜神经细胞，而是定位于视网膜穆勒细胞。使用纯化的培养视网膜穆勒细胞可证实这种细胞类型特异性。
这些结果表明，在无明显全身恶性肿瘤的情况下，针对视网膜穆勒细胞的自身免疫反应可能导致缓慢进展的视力恶化。
