Steck P A, Schwartz B A, Rosendahl M S, Gray G R
J Biol Chem. 1978 Aug 25;253(16):5625-9.
Mycolic acids derived from the cell walls of Mycobacterium bovis BCG, Mycobacterium bovis Bovinus I, Mycobacterium smegmatis, and Mycobacterium tuberculosis H37Rv have been fractionated as their p-bromophenacyl esters by a two-step high performance liquid chromatographic procedure: 1) adsorption chromatography on 10-micrometer particle size silica gel, and 2) reverse phase partition chromatography on a 10-micrometer particle size support containing a C18 bonded phase. This procedure has resulted in the isolation of approximately 24 mycolic acids from each bacterium (very likely homologs of various mycolate types) instead of the two to four that have previously been described. The implication of these results on the previously determined structures of these fatty acids is discussed.
源自卡介苗、牛型结核杆菌I、耻垢分枝杆菌和结核分枝杆菌H37Rv细胞壁的分枝菌酸，已通过两步高效液相色谱法分离为它们的对溴苯甲酰酯：1）在粒径为10微米的硅胶上进行吸附色谱法，以及2）在含有C18键合相的粒径为10微米的载体上进行反相分配色谱法。该方法已从每种细菌中分离出约24种分枝菌酸（很可能是各种分枝菌酸盐类型的同系物），而不是之前描述的两到四种。讨论了这些结果对先前确定的这些脂肪酸结构的影响。
