Cage G D
Clinical and Reference Microbiology Laboratory, Arizona Department of Health Services, Phoenix 85007.
J Clin Microbiol. 1992 Sep;30(9):2402-7. doi: 10.1128/jcm.30.9.2402-2407.1992.
An important class of fatty acids contained in the cell envelopes of Mycobacterium organisms is the group of high-molecular-weight, long-chain, alpha-branched, beta-hydroxylated mycolic acids. By using standard saponification techniques and derivatization of the acids to their p-bromophenacyl esters, it is possible to differentiate them by high-performance liquid chromatography. Mycolic acid chromatograms of 63 clinical isolates of Mycobacterium gordonae were compared with conventional biochemical methods for identification. The data show two distinct pattern types for this species, only one of which has been elaborated in the literature by using this protocol. Laboratory workers who intend to use this method as a clinical tool need to be aware that these two pattern types exist.
分枝杆菌生物体细胞膜中所含的一类重要脂肪酸是高分子量、长链、α-分支、β-羟基化的分枝菌酸。通过使用标准皂化技术并将这些酸衍生化为对溴苯甲酰酯，可以通过高效液相色谱对它们进行区分。将63株戈登分枝杆菌临床分离株的分枝菌酸色谱图与传统生化鉴定方法进行了比较。数据显示该菌种有两种不同的图谱类型，其中只有一种在文献中通过该方案进行了阐述。打算将此方法用作临床工具的实验室工作人员需要意识到这两种图谱类型的存在。
