与四氮唑还原法相比，丙酮酸发酵法在D群链球菌鉴别中的应用。
Use of pyruvate fermentation compared with tetrazolium reduction in the differentiation of group D streptococci.
作者信息
Waitkins S A
出版信息
J Clin Pathol. 1978 Jul;31(7):692-5. doi: 10.1136/jcp.31.7.692.
The reduction of 2, 3, 5 triphenyltetrazolium chloride in the original medium of Barnes (Journal of General Microbiology, 14, 57, 1956), and in a modified medium, was compared with the ability to ferment pyruvate as a means of separating Streptococcus faecalis from all other group D streptococci. The tetrazolium reduction test gave an occasional negative reaction with Str. faecalis strains. In addition, a number of strains gave a weakly positive reaction in the test, as did some strains of Str. faecium and Str. bovis. With some batches of tetrazolium, these weak reactions with Str. faecalis were so frequent that interpretation of the results was difficult. On the other hand, all Str. faecalis strains, and no other group D streptococci, gave a positive pyruvate-fermentation reaction in 48 hours.
在巴恩斯的原始培养基（《普通微生物学杂志》，1956年，第14卷，第57页）以及改良培养基中，比较了2,3,5 - 氯化三苯基四氮唑的还原情况与发酵丙酮酸的能力，以此作为从所有其他D群链球菌中分离粪肠球菌的一种方法。四氮唑还原试验偶尔会对粪肠球菌菌株产生阴性反应。此外，一些菌株在该试验中呈弱阳性反应，一些屎肠球菌和牛链球菌菌株也是如此。使用某些批次的四氮唑时，粪肠球菌的这些弱反应非常频繁，以至于结果难以解释。另一方面，所有粪肠球菌菌株在48小时内都会产生丙酮酸发酵阳性反应，而其他D群链球菌则不会。
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