Di Alberti L, Ngui S L, Porter S R, Speight P M, Scully C M, Zakrewska J M, Williams I G, Artese L, Piattelli A, Teo C G
Department of Oral Medicine, Eastman Dental Institute and Hospital, London, United Kingdom.
J Infect Dis. 1997 Mar;175(3):703-7. doi: 10.1093/infdis/175.3.703.
A 210-bp DNA segment specific to the human herpesvirus 8 (HHV-8) genome was amplified by nested polymerase chain reaction from 10 of 14 archived oral biopsy samples of HIV-positive patients in London who had no evidence of oral Kaposi's sarcoma (KS). Various oral sites were represented. Oral tissues from 20 general dental patients not known to be HIV-infected were negative. When DNA sequences of these products were compared with sequences derived from 5 oral KS tissues of AIDS patients in London and 10 skin biopsies of Italian patients with Mediterranean KS (total number of positive tissues = 25), 11 were found to be unique. DNA and predicted peptide motifs of these sequences were also different from those in 28 of 36 HHV-8-positive lesions previously reported from American and African patients. HHV-8 is tropic for the oral mucosa of HIV-infected persons, and HHV-8 variants, though diverse, may be geographically restricted.
通过巢式聚合酶链反应，从伦敦14例无口腔卡波西肉瘤（KS）证据的HIV阳性患者的存档口腔活检样本中，有10例扩增出了一段210 bp的、特异于人疱疹病毒8型（HHV-8）基因组的DNA片段。样本涵盖了口腔的各个部位。20例未知感染HIV的普通牙科患者的口腔组织检测结果为阴性。将这些产物的DNA序列与来自伦敦艾滋病患者的5例口腔KS组织以及意大利地中海KS患者的10例皮肤活检样本（阳性组织总数 = 25）的序列进行比较时，发现其中11个序列是独特的。这些序列的DNA和预测的肽基序也与之前报道的来自美国和非洲患者的36例HHV-8阳性病变中的28例不同。HHV-8对HIV感染者的口腔黏膜具有嗜性，并且HHV-8变体尽管多样，但可能存在地域限制。
