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用于检测急性非细菌性流行性胃肠炎病毒诺沃克毒株及其抗体的固相微量滴定放射免疫测定法。

Solid-phase microtiter radioimmunoassay for detection of the Norwalk strain of acute nonbacterial, epidemic gastroenteritis virus and its antibodies.

作者信息

Greenberg H B, Wyatt R G, Valdesuso J, Kalica A R, London W T, Chanock R M, Kapikian A Z

出版信息

J Med Virol. 1978;2(2):97-108. doi: 10.1002/jmv.1890020204.

Abstract

The development of microtiter solid-phase radioimmunoassays for the detection of Norwalk antigen and its antibody is described. The tests are simple to perform and are sensitive and specific. The test for antigen can be used on crude stool filtrates and suspensions. Both tests are at least as sensitive as immune electron microscopy and more sensitive than immune adherence assay.

摘要

本文描述了用于检测诺沃克抗原及其抗体的微量滴定板固相放射免疫测定法的开发。这些测试操作简单,灵敏且特异。抗原检测可用于粗粪便滤液和悬液。两种测试至少与免疫电子显微镜一样灵敏,且比免疫黏附测定法更灵敏。

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