用流行性病毒性胃肠炎诺沃克病毒因子对黑猩猩进行实验性感染。

Experimental infection of chimpanzees with the Norwalk agent of epidemic viral gastroenteritis.

作者信息

Wyatt R G, Greenberg H B, Dalgard D W, Allen W P, Sly D L, Thornhill T S, Chanock R M, Kapikian A Z

出版信息

J Med Virol. 1978;2(2):89-96. doi: 10.1002/jmv.1890020203.

DOI:10.1002/jmv.1890020203

Abstract

A fecal filtrate of human origin containing the Norwalk agent of epidemic viral gastroenteritis was administered by stomach tube to chimpanzees in an attempt to induce diarrheal disease. Significant postchallenge serum antibody rises against Norwalk viral antigens were demonstrated in all animals using the techniques of immune electron microscopy and radioimmunoassay. In addition, viral antigens were detected in feces from five of nine animals using radioimmunoassay. Clinical illness characterized by diarrhea and/or vomiting did not occur. Infection was transmitted subsequently by feeding four additional chimpanzees a fecal filtrate prepared from one of the previously infected animals. Development of an antibody response in four animals and detection of viral antigen in two animals that received this passage filtrate indicated that viral replication had occurred in the absence of clinical illness. The availability of the chimpanzee as an experimental animal host susceptible to infection with the Norwalk agent should facilitate the study of epidemic viral gastroenteritis.

摘要

将含有流行性病毒性胃肠炎诺沃克病毒因子的人源粪便滤液经胃管给予黑猩猩，试图诱发腹泻病。使用免疫电子显微镜和放射免疫测定技术，在所有动物中均显示出攻毒后血清抗体针对诺沃克病毒抗原显著升高。此外，通过放射免疫测定在9只动物中的5只粪便中检测到病毒抗原。未出现以腹泻和/或呕吐为特征的临床疾病。随后，通过给另外4只黑猩猩喂食从先前感染的一只动物制备的粪便滤液来传播感染。接受这种传代滤液的4只动物产生抗体反应，2只动物检测到病毒抗原，这表明在没有临床疾病的情况下发生了病毒复制。黑猩猩作为易受诺沃克病毒因子感染的实验动物宿主，其可用性应有助于流行性病毒性胃肠炎的研究。