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本文引用的文献

1
Detection of Chlamydia trachomatis by the Gen-Probe AMPLIFIED Chlamydia Trachomatis Assay (AMP CT) in urine specimens from men and women and endocervical specimens from women.采用Gen-Probe衣原体核酸扩增检测法（AMP CT）检测男性和女性尿液标本以及女性宫颈内膜标本中的沙眼衣原体。
J Clin Microbiol. 1998 Feb;36(2):391-4. doi: 10.1128/JCM.36.2.391-394.1998.
2
Serotyping and genotyping of genital Chlamydia trachomatis isolates reveal variants of serovars Ba, G, and J as confirmed by omp1 nucleotide sequence analysis.通过omp1核苷酸序列分析证实，生殖道沙眼衣原体分离株的血清分型和基因分型揭示了血清型Ba、G和J的变体。
J Clin Microbiol. 1998 Feb;36(2):345-51. doi: 10.1128/JCM.36.2.345-351.1998.
3
Comparison of three commercially available amplification assays, AMP CT, LCx, and COBAS AMPLICOR, for detection of Chlamydia trachomatis in first-void urine.三种市售扩增检测方法（AMP CT、LCx和COBAS AMPLICOR）用于检测首次晨尿中沙眼衣原体的比较。
J Clin Microbiol. 1997 Oct;35(10):2628-33. doi: 10.1128/jcm.35.10.2628-2633.1997.
4
Molecular and mutation trends analyses of omp1 alleles for serovar E of Chlamydia trachomatis. Implications for the immunopathogenesis of disease.沙眼衣原体血清型E的omp1等位基因的分子与突变趋势分析。对疾病免疫发病机制的意义。
J Clin Invest. 1997 Feb 1;99(3):475-83. doi: 10.1172/JCI119182.
5
Differences in clinical manifestations of genital chlamydial infections related to serovars.与血清型相关的生殖系统衣原体感染临床表现的差异。
Genitourin Med. 1996 Aug;72(4):261-5.
6
RNA amplification by nucleic acid sequence-based amplification with an internal standard enables reliable detection of Chlamydia trachomatis in cervical scrapings and urine samples.通过基于核酸序列的扩增技术并结合内标进行RNA扩增，能够可靠地检测宫颈刮片和尿液样本中的沙眼衣原体。
J Clin Microbiol. 1996 Dec;34(12):3108-14. doi: 10.1128/jcm.34.12.3108-3114.1996.
7
Direct detection of Chlamydia trachomatis in urine specimens from symptomatic and asymptomatic men by using a rapid polymerase chain reaction assay.通过快速聚合酶链反应检测法直接检测有症状和无症状男性尿液标本中的沙眼衣原体。
J Clin Microbiol. 1993 May;31(5):1209-12. doi: 10.1128/jcm.31.5.1209-1212.1993.
8
Improved PCR sensitivity for direct genotyping of Chlamydia trachomatis serovars by using a nested PCR.通过使用巢式PCR提高沙眼衣原体血清型直接基因分型的PCR灵敏度。
J Clin Microbiol. 1994 Feb;32(2):528-30. doi: 10.1128/jcm.32.2.528-530.1994.
9
Direct detection and genotyping of Chlamydia trachomatis in cervical scrapes by using polymerase chain reaction and restriction fragment length polymorphism analysis.利用聚合酶链反应和限制性片段长度多态性分析对宫颈刮片中沙眼衣原体进行直接检测和基因分型。
J Clin Microbiol. 1993 May;31(5):1060-5. doi: 10.1128/jcm.31.5.1060-1065.1993.
10
Comparison of two panels of monoclonal antibodies for determination of Chlamydia trachomatis serovars.用于确定沙眼衣原体血清型的两组单克隆抗体的比较。
J Clin Microbiol. 1994 Dec;32(12):2968-74. doi: 10.1128/jcm.32.12.2968-2974.1994.

对尿液样本中的沙眼衣原体进行基因分型将有助于开展大规模的流行病学研究。

Genotyping of Chlamydia trachomatis in urine specimens will facilitate large epidemiological studies.

作者信息

Morré S A, Moes R, Van Valkengoed I, Boeke J P, van Eijk J T, Meijer C J, Van den Brule A J

机构信息

Section of Molecular Pathology, Department of Pathology, University Hospital Vrije Universiteit, 1081 HV, Amsterdam, The Netherlands.

出版信息

J Clin Microbiol. 1998 Oct;36(10):3077-8. doi: 10.1128/JCM.36.10.3077-3078.1998.

DOI:10.1128/JCM.36.10.3077-3078.1998
PMID:9738074
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC105118/
Abstract

The serovars of Chlamydia trachomatis-positive urine specimens (n = 81; as detected by PCR and ligase chain reaction) were successfully analyzed in 94% of cases by omp1 PCR-based RFLP analysis. The use of urine specimens and this simple and sensitive typing method will greatly facilitate epidemiological studies of C. trachomatis serovar distribution in asymptomatic C. trachomatis infections in both females and males.

摘要

通过基于omp1 PCR的限制性片段长度多态性分析，在94%的病例中成功分析了沙眼衣原体阳性尿液标本（n = 81；通过PCR和连接酶链反应检测）的血清型。尿液标本的使用以及这种简单且灵敏的分型方法将极大地促进对沙眼衣原体在男性和女性无症状沙眼衣原体感染中血清型分布的流行病学研究。