水中枯草芽孢杆菌孢子的热杀灭并非由氧化损伤所致。
Heat killing of Bacillus subtilis spores in water is not due to oxidative damage.
作者信息
Setlow B, Setlow P
机构信息
Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032, USA.
出版信息
Appl Environ Microbiol. 1998 Oct;64(10):4109-12. doi: 10.1128/AEM.64.10.4109-4112.1998.
Abstract
The heat resistance of wild-type spores of Bacillus subtilis or spores (termed alpha-beta-) lacking DNA protective alpha/beta-type small, acid-soluble spore proteins was not altered by anaerobiosis or high concentrations of the free radical scavenging agents ethanethiol and ethanedithiol. Heat-killed wild-type and alpha-beta- spores exhibited no increase in either protein carbonyl content or oxidized bases in DNA. These data strongly suggest that oxidative damage to spore macromolecules does not contribute significantly to spore killing by heat.
摘要
枯草芽孢杆菌野生型孢子或缺乏具有DNA保护作用的α/β型小酸溶性孢子蛋白的孢子（称为α-β-）的耐热性，不会因厌氧或高浓度的自由基清除剂乙硫醇和乙二硫醇而改变。经热灭活的野生型和α-β-孢子的蛋白质羰基含量或DNA中的氧化碱基均未增加。这些数据有力地表明，孢子大分子的氧化损伤对热致孢子死亡的作用并不显著。
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