与DNA结合的小的酸溶性蛋白质可保护枯草芽孢杆菌孢子免受干热杀灭。
Small, acid-soluble proteins bound to DNA protect Bacillus subtilis spores from killing by dry heat.
作者信息
Setlow B, Setlow P
机构信息
Department of Biochemistry, University of Connecticut Health Center, Farmington 06030, USA.
出版信息
Appl Environ Microbiol. 1995 Jul;61(7):2787-90. doi: 10.1128/aem.61.7.2787-2790.1995.
Abstract
Dry Bacillus subtilis spores lacking their two major DNA-binding proteins (small, acid-soluble proteins [SASP] alpha and beta) were much more sensitive to dry heat than were wild-type spores. Survivors of dry heat treatment of both wild-type and mutant spores exhibited a high frequency of mutations, and the DNA from the heated spores had increased numbers of single-strand breaks. These data indicate that SASP alpha and beta provide significant protection to spore DNA against the damaging effects of dry heat. This DNA damage may be in part depurination, and a purified alpha/beta-type SASP gave significant protection against dry heat-induced DNA depurination in vitro.
摘要
缺乏两种主要DNA结合蛋白（小的、酸溶性蛋白[SASP]α和β）的枯草芽孢杆菌干孢子比野生型孢子对干热更敏感。野生型和突变型孢子干热处理的存活者都表现出高频率的突变，并且加热孢子的DNA单链断裂数量增加。这些数据表明SASPα和β为孢子DNA提供了显著保护，使其免受干热的破坏作用。这种DNA损伤可能部分是脱嘌呤作用，并且纯化的α/β型SASP在体外对干热诱导的DNA脱嘌呤具有显著保护作用。
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