天蓝色链霉菌的20S蛋白酶体。
The 20S proteasome of Streptomyces coelicolor.
作者信息
Nagy I, Tamura T, Vanderleyden J, Baumeister W, De Mot R
机构信息
F. A. Janssens Laboratory of Genetics, Catholic University of Leuven, B-3001 Heverlee, Belgium.
出版信息
J Bacteriol. 1998 Oct;180(20):5448-53. doi: 10.1128/JB.180.20.5448-5453.1998.
Abstract
20S proteasomes were purified from Streptomyces coelicolor A3(2) and shown to be built from one alpha-type subunit (PrcA) and one beta-type subunit (PrcB). The enzyme displayed chymotrypsin-like activity on synthetic substrates and was sensitive to peptide aldehyde and peptide vinyl sulfone inhibitors and to the Streptomyces metabolite lactacystin. Characterization of the structural genes revealed an operon-like gene organization (prcBA) similar to Rhodococcus and Mycobacterium spp. and showed that the beta subunit is encoded with a 53-amino-acid propeptide which is removed during proteasome assembly. The upstream DNA region contains the conserved orf7 and an AAA ATPase gene (arc).
摘要
从天蓝色链霉菌A3(2)中纯化出20S蛋白酶体，结果表明其由一个α型亚基（PrcA）和一个β型亚基（PrcB）组成。该酶对合成底物表现出胰凝乳蛋白酶样活性，对肽醛和肽乙烯砜抑制剂以及链霉菌代谢物乳胞素敏感。结构基因的特征分析显示，其具有与红球菌属和分枝杆菌属类似的操纵子样基因组织（prcBA），并表明β亚基由一个53个氨基酸的前肽编码，该前肽在蛋白酶体组装过程中被去除。上游DNA区域包含保守的orf7和一个AAA ATP酶基因（arc）。
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