Gutacker M, Valsangiacomo C, Balmelli T, Bernasconi M V, Bouras C, Piffaretti J C
Istituto Cantonale Batteriosierologico, Lugano, Switzerland.
Res Microbiol. 1998 Jan;149(1):31-7. doi: 10.1016/s0923-2508(97)83621-2.
The involvement of spirochaetes, such as the aetiologic agent of Lyme borreliosis, Borrelia burgdorferi sensu lato, in Alzheimer's disease (AD), a common neuropathology, has been proposed by several groups in the past. In our laboratory, brains from 10 AD patients were analysed for the presence of B. burgdorferi sensu lato by both standard and nested PCR techniques based on various target regions, such as the hbb gene (encoding the histone-like protein HBb), the fla gene (flagellin), the rrl-rrf ribosomal intergenic spacer region and the rrs gene (encoding 16S rRNA). In addition, ELISA and Western blot tests for the detection of antibodies against spirochaetal antigens were performed on 27 sera from clinical AD patients. Using these methods, we did not obtain any evidence of the involvement of B. burgdorferi in Alzheimer's disease.
过去已有多个研究小组提出，诸如莱姆病的病原体伯氏疏螺旋体（Borrelia burgdorferi sensu lato）这类螺旋体，与常见神经病理学疾病阿尔茨海默病（AD）有关。在我们实验室，运用基于不同靶区域（如编码类组蛋白HBb的hbb基因、鞭毛蛋白的fla基因、rrl - rrf核糖体基因间隔区以及编码16S rRNA的rrs基因）的标准PCR和巢式PCR技术，对10例AD患者的大脑进行分析，以检测伯氏疏螺旋体（Borrelia burgdorferi sensu lato）的存在情况。此外，对27例临床AD患者的血清进行了ELISA和蛋白质印迹试验，以检测抗螺旋体抗原的抗体。运用这些方法，我们未获得任何证据表明伯氏疏螺旋体（Borrelia burgdorferi）与阿尔茨海默病有关。
