Meert K L, Ofenstein J P, Sarnaik A P
Department of Pediatrics, Children's Hospital of Michigan, Wayne State University School of Medicine, Detroit 48201, USA.
Ann Clin Lab Sci. 1998 Sep-Oct;28(5):283-8.
Cell mediated immunity is suppressed following traumatic injury. The Objective is to determine whether there is a shift from T helper type 1 (TH1) to TH2 cell cytokine production following mechanical trauma in a rat model.
Male Sprague-Dawley rats were anesthetized and subjected to bilateral femur fractures or sham injury. Spleens were removed 3 days later. T cell proliferation and cytokine production were stimulated by culturing spleen cells with the T cell mitogen concanavalin A (con A). Interleukin-2 (IL-2), interferon-gamma (IFN-gamma), IL-10 and IL-4 concentrations were measured in spleen cell supernatants using enzyme linked immunosorbent assays.
Con A-induced spleen cell proliferation was decreased in traumatized rats compared to controls (p < 0.05). Spleen cell supernatant concentrations of the TH1 cytokines IL-2 and IFN-gamma were decreased in the trauma group (p < 0.05). Supernatant concentrations of the TH2 cytokine IL-10 were also decreased in traumatized rats (p < 0.01). The IL-4 concentrations were below the detection limit (< 15 pg/mL) in all cell supernatants.
Mechanical tissue injury leads to generalized suppression of T helper cell cytokine production rather than a shift from TH1 to TH2 cell activity. Post-trauma cellular immunosuppression is not mediated via excess IL-10 production by TH2 cells.
创伤性损伤后细胞介导的免疫受到抑制。目的是确定在大鼠模型中机械性创伤后是否存在从1型辅助性T细胞（TH1）向TH2细胞细胞因子产生的转变。
将雄性斯普拉格-道利大鼠麻醉后造成双侧股骨骨折或假损伤。3天后取出脾脏。用T细胞丝裂原伴刀豆球蛋白A（con A）培养脾细胞，刺激T细胞增殖和细胞因子产生。使用酶联免疫吸附测定法测量脾细胞上清液中白细胞介素-2（IL-2）、干扰素-γ（IFN-γ）、IL-10和IL-4的浓度。
与对照组相比，创伤大鼠中con A诱导的脾细胞增殖减少（p < 0.05）。创伤组中TH1细胞因子IL-2和IFN-γ的脾细胞上清液浓度降低（p < 0.05）。创伤大鼠中TH2细胞因子IL-10的上清液浓度也降低（p < 0.01）。所有细胞上清液中的IL-4浓度均低于检测限（< 15 pg/mL）。
机械性组织损伤导致辅助性T细胞细胞因子产生普遍受到抑制，而非从TH1向TH2细胞活性的转变。创伤后细胞免疫抑制不是通过TH2细胞过量产生IL-10介导的。
