Kussmaul M, Wilimzig M, Bock E
Institut fur Bodenkunde, Universitat Hamburg, 20146 Hamburg, Germany.
Appl Environ Microbiol. 1998 Nov;64(11):4530-2. doi: 10.1128/AEM.64.11.4530-4532.1998.
Methanotrophs were present in 48 of 225 stone samples which were removed from 19 historical buildings in Germany and Italy. The average cell number of methanotrophs was 20 CFU per g of stone, and their activities ranged between 11 and 42 pmol of CH4 g of stone-1 day-1. Twelve strains of methane-oxidizing bacteria were isolated. They belonged to the type II methanotrophs of the genera Methylocystis, Methylosinus, and Methylobacterium. In masonry, growth substrates like methane or methanol are available in very low concentrations. To determine if methane could be produced by the stone at rates sufficient to support growth of methanotrophs, methane production by stone samples under nonoxic conditions was examined. Methane production of 0.07 to 215 nmol of CH4 g of stone-1 day-1 was detected in 23 of 47 stone samples examined. This indicated the presence of the so-called "mini-methane"-producing bacteria and/or methanogenic archaea. Methanotrophs occurred in nearly all samples which showed methane production. This finding indicated that methanotrophs depend on biogenic methane production in or on stone surfaces of historical buildings.
从德国和意大利的19座历史建筑中采集的225个石材样本中，有48个样本存在甲烷氧化菌。甲烷氧化菌的平均细胞数为每克石材20 CFU，其活性范围在每克石材每天11至42 pmol的CH₄之间。分离出了12株甲烷氧化细菌。它们属于甲基孢囊菌属、甲基弯曲菌属和甲基杆菌属的II型甲烷氧化菌。在砖石结构中，甲烷或甲醇等生长底物的浓度非常低。为了确定石材产生甲烷的速率是否足以支持甲烷氧化菌的生长，研究了无氧条件下石材样本的甲烷产生情况。在所检测的47个石材样本中，有23个检测到甲烷产生量为每克石材每天0.07至215 nmol的CH₄。这表明存在所谓的“微型甲烷”产生细菌和/或产甲烷古菌。几乎所有显示有甲烷产生的样本中都存在甲烷氧化菌。这一发现表明，甲烷氧化菌依赖于历史建筑石材表面或内部的生物源甲烷产生。
