van den Broek F A, Beynen A C
Small Animal Centre, Agricultural University Wageningen, The Netherlands.
Lab Anim. 1998 Oct;32(4):483-91. doi: 10.1258/002367798780599758.
Dystrophic cardiac calcification (DCC) is often found in DBA/2 mice, reportedly in association with low plasma magnesium levels in this mouse strain. It was hypothesized that high-phosphorus diets and low-magnesium diets that are known to lower plasma magnesium concentrations would promote the development of DCC. DBA/2 mice were fed diets with either low-magnesium (0.02%, w/w) or high-phosphorus (0.8%) concentrations or a combination of the two variables. NMRI mice were given either a low- (0.2%) or high- (0.6%) phosphorus diet. Female, but not male, NMRI mice accumulated calcium in the heart when fed the high-phosphorus diet; neither gender developed kidney calcification. DBA/2 mice with either a low-magnesium or a high-phosphorus intake developed marked cardiac calcifications. The combination of low-magnesium and high-phosphorus intake caused severe calcification of the heart, kidney and tongue. Increasing the dietary magnesium content (0.08%) and reducing phosphorus (0.2%) did not fully prevent cardiac calcification, but reduced heart calcium concentrations in male DBA/2 mice. It is concluded that diets for DCC-susceptible mice should contain adequate amounts of magnesium and low, but sufficient amounts of phosphorus in order not to additionally stimulate cardiac calcification.
营养不良性心脏钙化（DCC）常见于DBA/2小鼠中，据报道，这与该小鼠品系的血浆镁水平较低有关。据推测，已知会降低血浆镁浓度的高磷饮食和低镁饮食会促进DCC的发展。给DBA/2小鼠喂食低镁（0.02%，w/w）或高磷（0.8%）浓度的饮食或这两个变量的组合。给NMRI小鼠喂食低磷（0.2%）或高磷（0.6%）饮食。雌性而非雄性NMRI小鼠在喂食高磷饮食时心脏会积累钙；两种性别均未出现肾脏钙化。摄入低镁或高磷的DBA/2小鼠出现明显的心脏钙化。低镁和高磷摄入的组合导致心脏、肾脏和舌头严重钙化。增加饮食中的镁含量（0.08%）并降低磷含量（0.2%）并不能完全预防心脏钙化，但可降低雄性DBA/2小鼠心脏中的钙浓度。结论是，易患DCC的小鼠饮食应含有适量的镁和低但足够量的磷，以免额外刺激心脏钙化。
