Hu Z L, Zhang J P, Qian D H, Lin W, Xie W F, Zhang X R, Chen W Z
Research Laboratory of Natural and Synthetic Drugs, College of Pharmacy, Second Military Medical University, Shanghai, China.
Zhongguo Yao Li Xue Bao. 1996 May;17(3):259-61.
To investigate the mechanisms of anti-inflammatory effect of matrine (Mat), its effects on mouse splenocyte proliferation, and release of interleukin-1 (IL-1) and interleukin-6 (IL-6) from mouse peritoneal macrophages.
Splenocyte proliferation was assayed by [3H] TdR incorporation. IL-1 and IL-6 activities were measured by thymocyte proliferation assay and B9 cell proliferation MTT colorimetric method, respectively.
Mat (125-500 mg.L-1) obviously inhibited concanavalin A (Con A, 5 mg.L-1)- and lipopolysaccarides (LPS, 10 mg.L-1)-induced splenocyte proliferation and LPS-induced release of IL-1 and IL-6 from mouse peritoneal macrophages.
Mat inhibited splenocyte proliferation and release of IL-1 and IL-6 in vitro.
研究苦参碱（Mat）的抗炎作用机制、对小鼠脾细胞增殖的影响以及对小鼠腹腔巨噬细胞白细胞介素-1（IL-1）和白细胞介素-6（IL-6）释放的影响。
采用[3H]TdR掺入法检测脾细胞增殖。分别采用胸腺细胞增殖试验和B9细胞增殖MTT比色法检测IL-1和IL-6活性。
Mat（125 - 500 mg.L-1）明显抑制刀豆蛋白A（Con A，5 mg.L-1）和脂多糖（LPS，10 mg.L-1）诱导的脾细胞增殖以及LPS诱导的小鼠腹腔巨噬细胞IL-1和IL-6释放。
Mat在体外抑制脾细胞增殖以及IL-1和IL-6的释放。
