• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Synthetic lethal interactions with conditional poly(A) polymerase alleles identify LCP5, a gene involved in 18S rRNA maturation.与条件性聚腺苷酸聚合酶等位基因的合成致死相互作用鉴定出LCP5，一个参与18S核糖体RNA成熟的基因。
RNA. 1998 Nov;4(11):1357-72. doi: 10.1017/s1355838298980955.
2
Rcl1p, the yeast protein similar to the RNA 3'-phosphate cyclase, associates with U3 snoRNP and is required for 18S rRNA biogenesis.Rcl1p是一种与RNA 3'-磷酸环化酶相似的酵母蛋白，它与U3小核仁核糖核蛋白（U3 snoRNP）结合，是18S核糖体RNA（rRNA）生物合成所必需的。
EMBO J. 2000 May 2;19(9):2115-26. doi: 10.1093/emboj/19.9.2115.
3
A nuclear 3'-5' exonuclease involved in mRNA degradation interacts with Poly(A) polymerase and the hnRNA protein Npl3p.一种参与mRNA降解的核3'-5'核酸外切酶与聚腺苷酸聚合酶及核不均一RNA蛋白Npl3p相互作用。
Mol Cell Biol. 2000 Jan;20(2):604-16. doi: 10.1128/MCB.20.2.604-616.2000.
4
Mutational analysis of an essential binding site for the U3 snoRNA in the 5' external transcribed spacer of yeast pre-rRNA.酵母前体rRNA 5' 外部转录间隔区中U3 snoRNA必需结合位点的突变分析。
Nucleic Acids Res. 1994 Nov 25;22(23):5139-47. doi: 10.1093/nar/22.23.5139.
5
Mutational analysis of an essential binding site for the U3 snoRNA in the 5' external transcribed spacer of yeast pre-rRNA.酵母前体rRNA 5' 外部转录间隔区中U3 snoRNA关键结合位点的突变分析。
Nucleic Acids Res. 1994 Oct 11;22(20):4057-65. doi: 10.1093/nar/22.20.4057.
6
Two mutant forms of the S1/TPR-containing protein Rrp5p affect the 18S rRNA synthesis in Saccharomyces cerevisiae.含S1/TPR的蛋白质Rrp5p的两种突变形式影响酿酒酵母中的18S rRNA合成。
RNA. 1998 Dec;4(12):1636-52. doi: 10.1017/s1355838298981511.
7
Processing of 20S pre-rRNA to 18S ribosomal RNA in yeast requires Rrp10p, an essential non-ribosomal cytoplasmic protein.在酵母中，将20S前体核糖体RNA加工成18S核糖体RNA需要Rrp10p，它是一种必需的非核糖体细胞质蛋白。
EMBO J. 2001 Aug 1;20(15):4204-13. doi: 10.1093/emboj/20.15.4204.
8
Bypassing the rRNA processing endonucleolytic cleavage at site A2 in Saccharomyces cerevisiae.绕过酿酒酵母中位点A2处的核糖体RNA加工内切核酸酶切割。
RNA. 2000 Nov;6(11):1498-508. doi: 10.1017/s1355838200000558.
9
Fal1p is an essential DEAD-box protein involved in 40S-ribosomal-subunit biogenesis in Saccharomyces cerevisiae.Fal1p是一种参与酿酒酵母40S核糖体亚基生物合成的必需DEAD盒蛋白。
Mol Cell Biol. 1997 Dec;17(12):7283-94. doi: 10.1128/MCB.17.12.7283.
10
RNA14 and RNA15 proteins as components of a yeast pre-mRNA 3'-end processing factor.RNA14和RNA15蛋白作为酵母前体mRNA 3'末端加工因子的组成部分。
Science. 1994 Dec 9;266(5191):1702-5. doi: 10.1126/science.7992054.

引用本文的文献

1
The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.TOR 依赖性磷酸化蛋白质组和细胞蛋白质合成的调控。
EMBO J. 2021 Aug 16;40(16):e107911. doi: 10.15252/embj.2021107911. Epub 2021 Jul 23.
2
Sgd1 is an MIF4G domain-containing cofactor of the RNA helicase Fal1 and associates with the 5' domain of the 18S rRNA sequence.Sgd1 是 RNA 解旋酶 Fal1 的 MIF4G 结构域包含的辅助因子，与 18S rRNA 序列的 5' 结构域结合。
RNA Biol. 2020 Apr;17(4):539-553. doi: 10.1080/15476286.2020.1716540. Epub 2020 Jan 29.
3
Sas10 controls ribosome biogenesis by stabilizing Mpp10 and delivering the Mpp10-Imp3-Imp4 complex to nucleolus.Sas10 通过稳定 Mpp10 并将 Mpp10-Imp3-Imp4 复合物递送至核仁来控制核糖体的生物发生。
Nucleic Acids Res. 2019 Apr 8;47(6):2996-3012. doi: 10.1093/nar/gkz105.
4
Human AATF/Che-1 forms a nucleolar protein complex with NGDN and NOL10 required for 40S ribosomal subunit synthesis.人类AATF/Che-1与40S核糖体亚基合成所需的NGDN和NOL10形成一种核仁蛋白复合物。
Nucleic Acids Res. 2016 Nov 16;44(20):9803-9820. doi: 10.1093/nar/gkw790. Epub 2016 Sep 5.
5
C1D family proteins in coordinating RNA processing, chromosome condensation and DNA damage response.C1D家族蛋白在协调RNA加工、染色体凝聚和DNA损伤反应方面的作用。
Cell Div. 2016 Mar 9;11:2. doi: 10.1186/s13008-016-0014-5. eCollection 2016.
6
Stage-specific assembly events of the 6-MDa small-subunit processome initiate eukaryotic ribosome biogenesis.特定阶段的 6-MDa 小亚基加工体组装事件启动真核核糖体生物发生。
Nat Struct Mol Biol. 2015 Nov;22(11):920-3. doi: 10.1038/nsmb.3111. Epub 2015 Oct 19.
7
Identification and Expression Analysis of Ribosome Biogenesis Factor Co-orthologs in Solanum lycopersicum.番茄核糖体生物发生因子共直系同源物的鉴定与表达分析
Bioinform Biol Insights. 2015 Feb 4;9:1-17. doi: 10.4137/BBI.S20751. eCollection 2015.
8
Ribosome biogenesis in the yeast Saccharomyces cerevisiae.酵母酿酒酵母中的核糖体生物发生。
Genetics. 2013 Nov;195(3):643-81. doi: 10.1534/genetics.113.153197.
9
The small subunit processome in ribosome biogenesis—progress and prospects.核糖体生物发生中的小亚基加工体——进展与展望。
Wiley Interdiscip Rev RNA. 2011 Jan-Feb;2(1):1-21. doi: 10.1002/wrna.57.
10
The C-terminal region of the exosome-associated protein Rrp47 is specifically required for box C/D small nucleolar RNA 3'-maturation.外切体相关蛋白 Rrp47 的 C 端区域特异性地参与 box C/D 小核仁 RNA 3'-成熟过程。
J Biol Chem. 2011 Feb 11;286(6):4535-43. doi: 10.1074/jbc.M110.162826. Epub 2010 Dec 6.

本文引用的文献

1
Fal1p is an essential DEAD-box protein involved in 40S-ribosomal-subunit biogenesis in Saccharomyces cerevisiae.Fal1p是一种参与酿酒酵母40S核糖体亚基生物合成的必需DEAD盒蛋白。
Mol Cell Biol. 1997 Dec;17(12):7283-94. doi: 10.1128/MCB.17.12.7283.
2
Mechanism and regulation of mRNA polyadenylation.mRNA 多聚腺苷酸化的机制与调控
Genes Dev. 1997 Nov 1;11(21):2755-66. doi: 10.1101/gad.11.21.2755.
3
Hrp1, a sequence-specific RNA-binding protein that shuttles between the nucleus and the cytoplasm, is required for mRNA 3'-end formation in yeast.Hrp1是一种序列特异性RNA结合蛋白，在细胞核和细胞质之间穿梭，是酵母中mRNA 3'末端形成所必需的。
Genes Dev. 1997 Oct 1;11(19):2545-56. doi: 10.1101/gad.11.19.2545.
4
Mpp10p, a U3 small nucleolar ribonucleoprotein component required for pre-18S rRNA processing in yeast.Mpp10p，一种酵母中前体18S核糖体RNA加工所需的U3小核仁核糖核蛋白组分。
Mol Cell Biol. 1997 Oct;17(10):5803-12. doi: 10.1128/MCB.17.10.5803.
5
A multisubunit 3' end processing factor from yeast containing poly(A) polymerase and homologues of the subunits of mammalian cleavage and polyadenylation specificity factor.一种来自酵母的多亚基3'端加工因子，包含多聚腺苷酸聚合酶以及哺乳动物切割和聚腺苷酸化特异性因子亚基的同源物。
EMBO J. 1997 Aug 1;16(15):4727-37. doi: 10.1093/emboj/16.15.4727.
6
A review of phenotypes in Saccharomyces cerevisiae.酿酒酵母表型综述。
Yeast. 1997 Sep 30;13(12):1099-133. doi: 10.1002/(SICI)1097-0061(19970930)13:12<1099::AID-YEA177>3.0.CO;2-7.
7
eIF4G dramatically enhances the binding of eIF4E to the mRNA 5'-cap structure.真核生物翻译起始因子4G（eIF4G）显著增强真核生物翻译起始因子4E（eIF4E）与mRNA 5'帽结构的结合。
J Biol Chem. 1997 Aug 29;272(35):21677-80. doi: 10.1074/jbc.272.35.21677.
8
Translation initiation factor eIF4G mediates in vitro poly(A) tail-dependent translation.翻译起始因子eIF4G介导体外多聚腺苷酸尾依赖性翻译。
Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):9046-51. doi: 10.1073/pnas.94.17.9046.
9
The major yeast poly(A)-binding protein is associated with cleavage factor IA and functions in premessenger RNA 3'-end formation.主要的酵母聚腺苷酸结合蛋白与切割因子IA相关，并在信使前体RNA 3'末端形成过程中发挥作用。
Proc Natl Acad Sci U S A. 1997 Jul 22;94(15):7897-902. doi: 10.1073/pnas.94.15.7897.
10
Starting at the beginning, middle, and end: translation initiation in eukaryotes.从起始、中间和结尾开始：真核生物中的翻译起始
Cell. 1997 Jun 13;89(6):831-8. doi: 10.1016/s0092-8674(00)80268-8.

与条件性聚腺苷酸聚合酶等位基因的合成致死相互作用鉴定出LCP5，一个参与18S核糖体RNA成熟的基因。

Synthetic lethal interactions with conditional poly(A) polymerase alleles identify LCP5, a gene involved in 18S rRNA maturation.

作者信息

Wiederkehr T, Prétôt R F, Minvielle-Sebastia L

机构信息

Department of Cell Biology, Biozentrum, University of Basel, Switzerland.

出版信息

RNA. 1998 Nov;4(11):1357-72. doi: 10.1017/s1355838298980955.

DOI:10.1017/s1355838298980955
PMID:9814757
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1369709/
Abstract

To identify new genes involved in 3'-end formation of mRNAs in Saccharomyces cerevisiae, we carried out a screen for synthetic lethal mutants with the conditional poly(A) polymerase allele, pap1-7. Five independent temperature-sensitive mutations called Icp1 to Icp5 (for lethal with conditional pap1 allele) were isolated. Here, we describe the characterization of the essential gene LCP5 which codes for a protein with a calculated molecular mass of 40.8 kD. Unexpectedly, we found that mutations in LCP5 caused defects in pre-ribosomal RNA (pre-rRNA) processing, whereas mRNA 3'-end formation in vitro was comparable to wild-type. Early cleavage steps (denoted A0 to A2) that lead to the production of mature 18S rRNA were impaired. In vivo depletion of Lcp5p also inhibited pre-rRNA processing. As a consequence, mutant and depleted cells showed decreased levels of polysomes compared to wild-type cells. Indirect immunofluorescence indicated a predominant localization of Lcp5p in the nucleolus. In addition, antibodies directed against Lcp5p specifically immunoprecipitated the yeast U3 snoRNA snR17, suggesting that the protein is directly involved in pre-rRNA processing.

摘要

为了鉴定酿酒酵母中参与mRNA 3'末端形成的新基因，我们对与条件性多聚腺苷酸聚合酶等位基因pap1-7合成致死的突变体进行了筛选。分离出了五个独立的温度敏感突变，分别称为Icp1至Icp5（与条件性pap1等位基因致死）。在此，我们描述了必需基因LCP5的特征，该基因编码一种计算分子量为40.8 kD的蛋白质。出乎意料的是，我们发现LCP5中的突变导致核糖体前体RNA（pre-rRNA）加工缺陷，而体外mRNA 3'末端形成与野生型相当。导致成熟18S rRNA产生的早期切割步骤（标记为A0至A2）受到损害。体内Lcp5p的缺失也抑制了pre-rRNA加工。因此，与野生型细胞相比，突变体和缺失细胞中的多核糖体水平降低。间接免疫荧光表明Lcp5p主要定位于核仁。此外，针对Lcp5p的抗体特异性免疫沉淀酵母U3 snoRNA snR17，表明该蛋白质直接参与pre-rRNA加工。