用于鉴定细菌性角膜炎患者病原体的16S核糖体DNA分型
16S ribosomal DNA typing for identification of pathogens in patients with bacterial keratitis.
作者信息
Knox C M, Cevellos V, Dean D
机构信息
Francis I. Proctor Foundation, Division of Infectious Disease, University of California at San Francisco School of Medicine, San Francisco, California, USA.
出版信息
J Clin Microbiol. 1998 Dec;36(12):3492-6. doi: 10.1128/JCM.36.12.3492-3496.1998.
Abstract
The identification of pathogens in patients with bacterial keratitis remains problematic because standard diagnostic tests are negative for 40 to 60% of patients. A cross-sectional study was undertaken to determine if PCR and sequence analysis of 16S ribosomal DNA (rDNA) could be used to detect bacterial pathogens in patients with keratitis. Corneal specimens were collected for culture and rDNA typing. Variable segments of each rDNA specimen were amplified by PCR, sequenced, and aligned with the sequences in GenBank. Eleven patients had microbiologically documented bacterial keratitis, while 17 patients had keratitis due to other causes. Nine (82%) of 11 bacterial keratitis patients were PCR positive; each sequencing result matched the culture results. Seventeen (100%) patients with nonbacterial keratitis were PCR negative. Our data suggest that 16S rDNA typing holds promise as a rapid alternative to culture for identifying pathogens in patients with bacterial keratitis.
摘要
对于细菌性角膜炎患者，病原体的鉴定仍然存在问题，因为标准诊断测试对40%至60%的患者呈阴性。开展了一项横断面研究，以确定16S核糖体DNA（rDNA）的PCR和序列分析是否可用于检测角膜炎患者的细菌病原体。收集角膜标本进行培养和rDNA分型。通过PCR扩增每个rDNA标本的可变片段，进行测序，并与GenBank中的序列比对。11例患者有微生物学记录的细菌性角膜炎，而17例患者的角膜炎由其他原因引起。11例细菌性角膜炎患者中有9例（82%）PCR呈阳性；每个测序结果与培养结果相符。17例（100%）非细菌性角膜炎患者PCR呈阴性。我们的数据表明，16S rDNA分型有望成为一种快速替代培养的方法，用于鉴定细菌性角膜炎患者的病原体。
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