Levey S B, Katz H R, Abrams D A, Hirschbein M J, Marsh M J
Krieger Eye Institute, Sinai Hospital of Baltimore, Maryland, USA.
Cornea. 1997 Jul;16(4):383-6.
To ascertain the importance of routine cultures and gram stains in the management of ulcerative keratitis.
We retrospectively reviewed 119 consecutive corneal ulcers seen at Sinai Hospital of Baltimore. Cultures were obtained of the corneal ulcer and of the lids and conjunctivae of both eyes. Gram stains were performed by the hospital microbiology department on corneal scrapings from each ulcer.
Positive corneal cultures were obtained from 56 eyes (47.1%). Initial antibiotic therapy was changed based on culture results in 14.3% of culture-positive eyes that demonstrated a worsening clinical course. Gram stains were negative in all cases. The sensitivity and specificity of the lid and conjunctival cultures were determined.
Corneal cultures are important in the management of ulcerative keratitis. Lid and conjunctival cultures have low sensitivity and specificity.
确定常规培养和革兰氏染色在溃疡性角膜炎治疗中的重要性。
我们回顾性分析了巴尔的摩西奈医院连续收治的119例角膜溃疡病例。对角膜溃疡以及双眼的眼睑和结膜进行培养。医院微生物科对每个溃疡的角膜刮片进行革兰氏染色。
56只眼（47.1%）角膜培养呈阳性。在14.3%临床病程恶化的培养阳性眼中，初始抗生素治疗根据培养结果进行了调整。所有病例革兰氏染色均为阴性。测定了眼睑和结膜培养的敏感性和特异性。
角膜培养在溃疡性角膜炎治疗中很重要。眼睑和结膜培养的敏感性和特异性较低。
